Metcalf D, Robb L, Dunn A R, Mifsud S, Di Rago L
Walter and Eliza Hall Institute of Medical Research, Ludwig, Victoria, Australia.
Blood. 1996 Nov 15;88(10):3755-64.
The intraperitoneal injection into mice of casein preparations containing bacteria induced a rapid accumulation of neutrophils within 3 hours due to selective release of mature cells from the bone marrow. Significant increases in the concentrations of granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF) occurred in the peritoneal cavity during the process, but the intraperitoneal injection of neither CSF induced a significant accumulation of neutrophils and the coinjection of G-CSF and casein failed to enhance the neutrophil response. The lack of involvement of either CSF in the neutrophil migration was confirmed by the development of typical neutrophil exudates when casein was injected into mice with inactivation of the genes encoding GM-CSF, G-CSF, or the beta-common chain of the GM-CSF receptor. However, preinjection of G-CSF increased the number of marrow neutrophils available for migration and did result in increased numbers of neutrophils in the peritoneal cavity after casein injection. Typical eosinophil inflammatory responses to the injection of casein or thioglycollate occurred in GM-CSF -/ -mice but not in beta c -/- mice, suggesting that interleukin-5 was necessary for this response.
给小鼠腹腔注射含有细菌的酪蛋白制剂，由于骨髓中成熟细胞的选择性释放，3小时内中性粒细胞迅速积聚。在此过程中，腹腔内粒细胞-巨噬细胞集落刺激因子（GM-CSF）和粒细胞集落刺激因子（G-CSF）的浓度显著增加，但单独腹腔注射这两种集落刺激因子均未引起中性粒细胞的显著积聚，且G-CSF与酪蛋白联合注射也未能增强中性粒细胞反应。当将酪蛋白注射到编码GM-CSF、G-CSF或GM-CSF受体β共同链的基因失活的小鼠体内时，出现了典型的中性粒细胞渗出物，这证实了两种集落刺激因子均未参与中性粒细胞的迁移。然而，预先注射G-CSF增加了可供迁移的骨髓中性粒细胞数量，并且在注射酪蛋白后确实导致腹腔内中性粒细胞数量增加。GM-CSF - / -小鼠对注射酪蛋白或巯基乙酸盐出现典型的嗜酸性粒细胞炎症反应，但βc - / -小鼠未出现，这表明白细胞介素-5对该反应是必需的。
