Isoai A, Kawahara H, Okazaki Y, Shizuri Y
Marine Biotechnology Institute Co. Ltd., Shimizu Laboratory, Shizuoka, Japan.
Gene. 1996 Oct 10;175(1-2):95-100. doi: 10.1016/0378-1119(96)00130-8.
An intronless gene encoding a putative G protein-coupled receptor was isolated from the genomic library of barnacle Balanus amphitrite Darwin, with probes obtained from degenerate polymerase chain reaction (PCR) primers used to amplify putative transmembrane regions. The cloned genome DNA specifies an open reading frame of 1431 bp encoding 476 amino acids with seven hydrophobic transmembrane (TM)-spanning regions. The predicted protein contains potential asparagine-linked glycosylation and serine/threonine phosphorylation sites in the N-terminal and intracellular loops, respectively. Moreover, the protein has a consensus G protein-binding motif (Ala-Ile-Ser-Leu-Asp-Arg-Tyr-Leu-Ala) in TM domain III. This receptor is most closely related to human alpha 2-adrenergic receptor with 36.9% identity in 409 amino acids overlap. It is also homologous to human serotonin1A (5HT), snail pond 5HT and mouse D2-dopamine receptors with 33-36% identities. Within TM regions among these biogenic amine receptors, the cloned receptor shows considerable amino acid homology with more than 40% overall identities.
从藤壶白脊藤壶达尔文的基因组文库中分离出一个无内含子基因，该基因编码一种假定的G蛋白偶联受体，所用探针来自用于扩增假定跨膜区域的简并聚合酶链反应（PCR）引物。克隆的基因组DNA指定了一个1431 bp的开放阅读框，编码476个氨基酸，具有七个疏水跨膜（TM）区域。预测的蛋白质在N端和细胞内环中分别含有潜在的天冬酰胺连接糖基化和丝氨酸/苏氨酸磷酸化位点。此外，该蛋白质在TM结构域III中具有一个共有G蛋白结合基序（Ala-Ile-Ser-Leu-Asp-Arg-Tyr-Leu-Ala）。该受体与人类α2-肾上腺素能受体关系最为密切，在409个氨基酸重叠区域中有36.9%的同一性。它也与人类5-羟色胺1A（5HT）、蜗牛5HT和小鼠D2-多巴胺受体同源，同一性为33-36%。在这些生物胺受体的TM区域内，克隆的受体显示出相当高的氨基酸同源性，总体同一性超过40%。
