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大肠杆菌中脂多糖A生物合成的首个关键步骤：lpxA基因序列

First committed step of lipid A biosynthesis in Escherichia coli: sequence of the lpxA gene.

作者信息

Coleman J, Raetz C R

机构信息

Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison 53706.

出版信息

J Bacteriol. 1988 Mar;170(3):1268-74. doi: 10.1128/jb.170.3.1268-1274.1988.

DOI:10.1128/jb.170.3.1268-1274.1988
PMID:3277952
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC210902/
Abstract

The min 4 region of the Escherichia coli genome contains genes (lpxA and lpxB) that encode proteins involved in lipid A biosynthesis. We have determined the sequence of 1,350 base pairs of DNA upstream of the lpxB gene. This fragment of DNA contains the complete coding sequence for the 28.0-kilodalton lpxA gene product and an upstream open reading frame capable of encoding a 17-kilodalton protein (ORF17). In addition there appears to be an additional open reading frame (ORF?) immediately upstream of ORF17. The initiation codon for lpxA is a GUG codon, and the start codon for ORF17 is apparently a UUG codon. The start and stop codons overlap between ORF? and ORF17, ORF17 and lpxA, and lpxA and lpxB. This overlap is suggestive of translational coupling and argues that the genes are cotranscribed. Crowell et al. (D.N. Crowell, W.S. Reznikoff, and C.R.H. Raetz, J. Bacteriol. 169:5727-5734, 1987) and Tomasiewicz and McHenry (H.G. Tomasiewicz and C.S. McHenry, J. Bacteriol. 169:5735-5744, 1987) have demonstrated that there are three similarly overlapping coding regions downstream of lpxB including dnaE, suggesting the existence of a complex operon of at least seven genes: 5'-ORF?-ORF17-lpxA-lpxB-ORF23-dnaE-ORF37-3 '.

摘要

大肠杆菌基因组的min 4区域包含一些基因（lpxA和lpxB），这些基因编码参与脂多糖A生物合成的蛋白质。我们已经确定了lpxB基因上游1350个碱基对的DNA序列。这段DNA片段包含28.0千道尔顿的lpxA基因产物的完整编码序列以及一个能够编码17千道尔顿蛋白质的上游开放阅读框（ORF17）。此外，在ORF17紧邻的上游似乎还有一个额外的开放阅读框（ORF？）。lpxA的起始密码子是一个GUG密码子，而ORF17的起始密码子显然是一个UUG密码子。ORF？与ORF17、ORF17与lpxA以及lpxA与lpxB之间的起始和终止密码子存在重叠。这种重叠暗示了翻译偶联，并表明这些基因是共转录的。Crowell等人（D.N. Crowell、W.S. Reznikoff和C.R.H. Raetz，《细菌学杂志》169:5727 - 5734，1987年）以及Tomasiewicz和McHenry（H.G. Tomasiewicz和C.S. McHenry，《细菌学杂志》169:5735 - 5744，1987年）已经证明，在lpxB下游有三个类似重叠的编码区域，包括dnaE，这表明存在一个至少由七个基因组成的复杂操纵子：5'-ORF?-ORF17-lpxA-lpxB-ORF23-dnaE-ORF37-3'。