Dvorak A M, Morgan E S
Department of Pathology, Beth Israel Hospital, Boston, MA 02215, USA.
Int Arch Allergy Immunol. 1996 Nov;111(3):238-44. doi: 10.1159/000237373.
Immature human mast cells, arising from cord blood mononuclear cells cultured in the presence of the c-kit ligand of human or murine origin, contain mixtures of morphologically immature and mature secretory granules. Use of a new cytochemical technique to localize histamine in ultrastructural samples (based on the affinity of the enzyme, histaminase, for its substrate, histamine) localized this amine to mature mast cell granules of all substructural patterns present, as well as to condensation foci appearing in immature cytoplasmic granules. This cytochemical evidence of histamine bound to condensation foci during granule building in developing mast cells is analogous to evidence obtained during granule recovery of degranulated human basophils and mast cells.
源自人或鼠源c-kit配体存在下培养的脐血单核细胞的未成熟人肥大细胞，含有形态学上未成熟和成熟分泌颗粒的混合物。使用一种新的细胞化学技术在超微结构样本中定位组胺（基于组胺酶对其底物组胺的亲和力），将这种胺定位到所有存在的亚结构模式的成熟肥大细胞颗粒以及未成熟细胞质颗粒中出现的凝聚灶。在发育中的肥大细胞颗粒形成过程中组胺与凝聚灶结合的这种细胞化学证据类似于在脱颗粒的人嗜碱性粒细胞和肥大细胞颗粒恢复过程中获得的证据。
