Novella I S, Elena S F, Moya A, Domingo E, Holland J J
Department of Biology, University of California, San Diego, La Jolla 92093-0116, USA.
Mol Gen Genet. 1996 Oct 28;252(6):733-8. doi: 10.1007/BF02173980.
The population dynamics of RNA viruses have an important influence on fitness variation and, in consequence, on the adaptative potential and virulence of this ubiquitous group of pathogens. Earlier work with vesicular stomatitis virus showed that large population transfers were reproducibly associated with fitness increases, whereas repeated transfers from plaque to plaque (genetic bottlenecks) lead to losses in fitness. We demonstrate here that repeated five-plaque to five-plaque passage series yield long-term fitness stability, except for occasional stochastic fitness jumps. Repeated five-plaque passages regularly alternating with two consecutive large population transmissions did not cause fitness losses, but did limit the size of fitness gains that would otherwise have occurred. These results underscore the profound effects of bottleneck transmissions in virus evolution.
RNA病毒的群体动态对适应性变异具有重要影响，进而影响这类普遍存在的病原体群体的适应潜力和毒力。早期针对水疱性口炎病毒的研究表明，大量群体传代可重复性地与适应性增加相关，而从噬菌斑到噬菌斑的反复传代（基因瓶颈）则导致适应性丧失。我们在此证明，除了偶尔的随机适应性跃升外，反复进行五噬菌斑到五噬菌斑的传代系列可产生长期适应性稳定性。反复进行五噬菌斑传代并定期与两次连续的大量群体传播交替进行，不会导致适应性丧失，但会限制原本可能出现的适应性增加幅度。这些结果强调了瓶颈传播在病毒进化中的深远影响。
