牛白血病病毒RNA依赖的DNA聚合酶的特性分析
Characterization of the RNA dependent DNA polymerase of bovine leukemia virus.
作者信息
Demirhan I, Altanerova V, Chandra A, Gerber T, Hofmann D, Altaner C, Chandra P
机构信息
Laboratory of Molecular Biology (ZBC), University Medical School, Frankfurt am Main, Germany.
出版信息
Anticancer Res. 1996 Sep-Oct;16(5A):2501-5.
The reverse transcriptase-RNA dependent DNA polymerase of Bovine Leukemia Virus (BLV) was isolated and characterized. The enzyme has a molecular weight of about 80kd and the isoelectric point is 7.6. The enzyme prefers magnesium, as a divalent cation using synthetic homopolymeric template primer poly (C) oligo (dG). Monoclonal antibodies directed against reverse transcriptase of human immunodeficiency virus type I (HIV-I) did not crossreact with the isolated polymerase.
牛白血病病毒（BLV）的逆转录酶 - 依赖RNA的DNA聚合酶被分离并进行了特性鉴定。该酶分子量约为80kd，等电点为7.6。使用合成的同聚模板引物聚（C）寡聚（dG）时，该酶优先选择镁作为二价阳离子。针对I型人类免疫缺陷病毒（HIV - I）逆转录酶的单克隆抗体与分离出的聚合酶无交叉反应。
Zotero 插件