Graves D C, Diglio C A, Ferrer J F
Am J Vet Res. 1977 Nov;38(11):1739-44.
An RNA-dependent DNA polymerase or reverse transcriptase has been demonstrated in highly purified bovine leukemia virus (BLV) particles. The viral enzyme responded very effectively to the exogenous template primer polyneucleotide (poly) (rA)-oligonucleotide (oligo) (dT). Unlike the reverse transcriptases of most mammalian C type RNA viruses, and of the ubliquitous foamy-like bovine syncytial virus, the BLV enzyme prefers magnesium rather than manganese for optimal activity. The identification of several other conditions required for optimal activity of the viral reverse transcriptase led to the development of a rapid, sensitive, semiquantitative assay, which is comparable in sensitivity to the syncytia-infectivity assay for the detection of BLV in supernatant fluids of monolayer cell cultures. However, the reverse transcriptase assay is not sufficiently reproducible for obtaining routine detection of BLV in short-term cultures of bovine peripheral blood lymphocytes. Therefore, this assay does not seem to provide an accurate method for the diagnosis of BL virus infection in cattle.
在高度纯化的牛白血病病毒（BLV）颗粒中已证实存在一种依赖RNA的DNA聚合酶或逆转录酶。该病毒酶对外源模板引物多聚核苷酸（poly）（rA）-寡核苷酸（oligo）（dT）反应非常有效。与大多数哺乳动物C型RNA病毒以及普遍存在的泡沫样牛合胞体病毒的逆转录酶不同，BLV酶在最佳活性时更倾向于镁而非锰。对病毒逆转录酶最佳活性所需的其他几种条件的鉴定导致了一种快速、灵敏的半定量检测方法的开发，该方法在检测单层细胞培养上清液中的BLV时，灵敏度与合胞体感染性检测相当。然而，逆转录酶检测在牛外周血淋巴细胞短期培养中对BLV进行常规检测时，重复性不够好。因此，该检测方法似乎不能为牛的BL病毒感染诊断提供准确方法。
