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Specific immunostaining of CCK cells by use of synthetic fragment antisera.

作者信息

Polak J M, Pearse A G, Szelke M, Bloom S R, Hudson D, Facer P, Buchan A M, Bryant M G, Christophodes N, MacIntyre I

出版信息

Experientia. 1977 Jun 15;33(6):762-3. doi: 10.1007/BF01944178.

DOI:10.1007/BF01944178
PMID:891742
Abstract

Antibodies to the central fragments 9-20 dodecapeptide sequence of CCK were used for specific immunostaining of the CCK cells of the mammalian gut. The use of high specific antibodies to synthetic fragment, essential when there is a possibility of immunochemical cross reactions between antisera and hormones of similar molecular structure provides the key to increased understanding of the nature and relationships of peptide hormones.

摘要

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1
Specific immunostaining of CCK cells by use of synthetic fragment antisera.
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Immunoreactive component resembling cholecystokinin octapeptide in intestine.
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Production of rabbit antibody specific for amino-terminal residues of cholecystokinin octapeptide (CCK-8) by selective suppression of cross-reactive antibody response.通过选择性抑制交叉反应性抗体反应制备针对胆囊收缩素八肽(CCK-8)氨基末端残基的兔抗体。
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引用本文的文献

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Cell Tissue Res. 1980;211(2):331-43. doi: 10.1007/BF00236453.
2
The location of VIP in the pancreas of man and rat.血管活性肠肽在人和大鼠胰腺中的定位。
Diabetologia. 1980 Jan;18(1):73-8. doi: 10.1007/BF01228307.
3
[The endocrine cells of the digestive system (author's transl)].消化系统的内分泌细胞(作者译)

本文引用的文献

1
Hormones of the gastrointestinal tract.胃肠道激素。
Br Med Bull. 1974 Jan;30(1):62-7. doi: 10.1093/oxfordjournals.bmb.a071169.
2
Bifunctional reagents as vapour- and liquid-phase fixatives for immunohistochemistry.双功能试剂作为免疫组织化学的气相和液相固定剂。
Histochem J. 1975 Mar;7(2):179-86. doi: 10.1007/BF01004561.
3
Immunohistochemical identification of the cholecystokinin cell in the intestinal mucosa.肠黏膜中胆囊收缩素细胞的免疫组织化学鉴定
Klin Wochenschr. 1982 Mar 1;60(5):213-27. doi: 10.1007/BF01728340.
4
Morphological variation of immunoreactive cells positive to cholecystokinin 33 (10-20) and gastrin 34 (1-15) in human duodenum.人十二指肠中对胆囊收缩素33(10 - 20)和胃泌素34(1 - 15)呈免疫反应阳性的细胞的形态学变化。
Cell Tissue Res. 1986;244(3):519-25. doi: 10.1007/BF00212529.
5
Ultrastructural localization of cholecystokinin in endocrine cells of the dog duodenum by the immunogold technique.用免疫金技术对犬十二指肠内分泌细胞中胆囊收缩素进行超微结构定位
Histochemistry. 1985;83(4):331-6. doi: 10.1007/BF00684379.
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Electron immunohistochemical evidence for the human intestinal I cell as the source of CCK.人类肠道I细胞作为胆囊收缩素来源的免疫组化证据。
Gut. 1978 May;19(5):403-7. doi: 10.1136/gut.19.5.403.
Gastroenterology. 1976 Apr;70(4):528-32.
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Gastrin (first of two parts).
N Engl J Med. 1975 Jun 19;292(25):1324-34. doi: 10.1056/NEJM197506192922505.
5
Identification of cholecystokinin-secreting cells.胆囊收缩素分泌细胞的鉴定。
Lancet. 1975 Nov 22;2(7943):1016-8. doi: 10.1016/s0140-6736(75)90297-4.