肽糖基化的构象影响：糖基化对蛋白质折叠速率影响的一种可能模型。
Conformational influences of glycosylation of a peptide: a possible model for the effect of glycosylation on the rate of protein folding.
作者信息
Live D H, Kumar R A, Beebe X, Danishefsky S J
机构信息
Laboratory for Nucleic Acid and Protein Structure, Sloan-Kettering Institute for Cancer Research, New York, NY 10021, USA.
出版信息
Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12759-61. doi: 10.1073/pnas.93.23.12759.
Abstract
Improved strategies for synthesis make it possible to expand the range of glycopeptides available for detailed conformational studies. The glycopeptide 1 was synthesized using a new solid phase synthesis of carbohydrates and a convergent coupling to peptide followed by deprotection. Its conformational properties were subjected to NMR analysis and compared with a control peptide 2 prepared by conventional solid phase methods. Whereas peptide 2 fails to manifest any appreciable secondary structure, the glycopeptide 1 does show considerable conformational bias suggestive of an equilibrium between an ordered and a random state. The implications of this ordering effect for the larger issue of protein folding are considered.
摘要
改进的合成策略使得扩大可用于详细构象研究的糖肽范围成为可能。糖肽1是通过一种新的碳水化合物固相合成法、与肽的收敛偶联然后脱保护来合成的。对其构象性质进行了核磁共振分析，并与通过传统固相方法制备的对照肽2进行了比较。肽2未能表现出任何明显的二级结构，而糖肽1确实显示出相当大的构象偏向，表明在有序状态和随机状态之间存在平衡。考虑了这种有序效应对于更大的蛋白质折叠问题的影响。
相似文献
1
Conformational influences of glycosylation of a peptide: a possible model for the effect of glycosylation on the rate of protein folding.肽糖基化的构象影响：糖基化对蛋白质折叠速率影响的一种可能模型。
Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12759-61. doi: 10.1073/pnas.93.23.12759.
2
A molecular basis for glycosylation-induced conformational switching.糖基化诱导构象转换的分子基础。
Chem Biol. 1998 Aug;5(8):427-37. doi: 10.1016/s1074-5521(98)90159-4.
3
Glycopeptide synthesis and the effects of glycosylation on protein structure and activity.糖肽合成以及糖基化对蛋白质结构和活性的影响。
Chembiochem. 2000 Nov 17;1(4):214-46. doi: 10.1002/1439-7633(20001117)1:4<214::AID-CBIC214>3.0.CO;2-B.
4
Conformational studies of the glycopeptide Ac-Tyr-[Man5GlcNAc-beta-(1-->4)GlcNAc-beta-(1-->Ndelta)]-Asn-Leu-Thr-Se r-OBz and the constituent peptide and oligosaccharide.糖肽Ac-Tyr-[甘露糖五糖-葡糖胺-β-(1→4)-葡糖胺-β-(1→Nδ)]-天冬酰胺-亮氨酸-苏氨酸-丝氨酸-苄酯以及组成它的肽和寡糖的构象研究
Carbohydr Res. 2000 Mar 10;324(4):242-54. doi: 10.1016/s0008-6215(99)00247-5.
5
A strategy for a convergent synthesis of N-linked glycopeptides on a solid support.一种在固相载体上收敛合成N-连接糖肽的策略。
Science. 1995 Jul 14;269(5221):202-4. doi: 10.1126/science.7618080.
6
Effects of glycosylation on peptide conformation: a synergistic experimental and computational study.糖基化对肽构象的影响：一项实验与计算协同研究
J Am Chem Soc. 2004 Jul 14;126(27):8421-5. doi: 10.1021/ja0496266.
7
Effect of N-linked glycosylation on glycopeptide and glycoprotein structure.N-连接糖基化对糖肽和糖蛋白结构的影响。
Curr Opin Chem Biol. 1999 Dec;3(6):643-9. doi: 10.1016/s1367-5931(99)00021-6.
8
Glycopeptide and glycoprotein synthesis involving unprotected carbohydrate building blocks.涉及未保护碳水化合物构建单元的糖肽和糖蛋白合成。
Med Res Rev. 2005 Nov;25(6):655-78. doi: 10.1002/med.20033.
9
Solid-phase glycosylation of peptide templates and on-bead MAS-NMR analysis: perspectives for glycopeptide libraries.肽模板的固相糖基化及珠上MAS-NMR分析：糖肽文库的前景
Chemistry. 2001 Aug 17;7(16):3584-91. doi: 10.1002/1521-3765(20010817)7:16<3584::aid-chem3584>3.0.co;2-z.
10
Conformational studies of oligosaccharides and glycopeptides: complementarity of NMR, X-ray crystallography, and molecular modelling.寡糖和糖肽的构象研究：核磁共振、X射线晶体学和分子建模的互补性
Chem Rev. 2002 Feb;102(2):371-86. doi: 10.1021/cr990368i.
引用本文的文献
1
Chromatograms and Mass Spectra of High-Mannose and Paucimannose -Glycans for Rapid Isomeric Identifications.高甘露糖型和寡甘露糖型聚糖的色谱图和质谱图用于快速鉴定异构体。
J Proteome Res. 2024 Mar 1;23(3):939-955. doi: 10.1021/acs.jproteome.3c00640. Epub 2024 Feb 16.
2
Deficiency of N-linked glycosylation impairs immune function of B7-H6.N 连接糖基化缺陷会损害 B7-H6 的免疫功能。
Front Immunol. 2023 Nov 15;14:1255667. doi: 10.3389/fimmu.2023.1255667. eCollection 2023.
3
Identification of the High Mannose -Glycan Isomers Undescribed by Conventional Multicellular Eukaryotic Biosynthetic Pathways.鉴定常规多细胞真核生物生物合成途径未描述的高甘露糖聚糖异构体。
Anal Chem. 2023 Jun 13;95(23):8789-8797. doi: 10.1021/acs.analchem.2c05599. Epub 2023 May 26.
4
Structural identification of N-glycan isomers using logically derived sequence tandem mass spectrometry.使用逻辑推导序列串联质谱法对N-聚糖异构体进行结构鉴定。
Commun Chem. 2021 Jun 17;4(1):92. doi: 10.1038/s42004-021-00532-z.
5
Improvement of the catalytic activity and thermostability of a hyperthermostable endoglucanase by optimizing N-glycosylation sites.通过优化N-糖基化位点提高超嗜热内切葡聚糖酶的催化活性和热稳定性。
Biotechnol Biofuels. 2020 Feb 26;13:30. doi: 10.1186/s13068-020-1668-4. eCollection 2020.
6
Calcitonin Receptor N-Glycosylation Enhances Peptide Hormone Affinity by Controlling Receptor Dynamics.降钙素受体 N-糖基化通过控制受体动力学增强肽激素亲和力。
J Mol Biol. 2020 Mar 27;432(7):1996-2014. doi: 10.1016/j.jmb.2020.01.028. Epub 2020 Feb 6.
7
GRASP depletion-mediated Golgi destruction decreases cell adhesion and migration via the reduction of α5β1 integrin.GRASP 耗竭介导的高尔基体破坏通过减少 α5β1 整联蛋白降低细胞黏附和迁移。
Mol Biol Cell. 2019 Mar 15;30(6):766-777. doi: 10.1091/mbc.E18-07-0462. Epub 2019 Jan 16.
8
Relative Quantification and Higher-Order Modeling of the Plasma Glycan Cancer Burden Ratio in Ovarian Cancer Case-Control Samples.卵巢癌病例对照样本中血浆聚糖癌症负担比率的相对定量和高阶建模
J Proteome Res. 2015 Oct 2;14(10):4394-401. doi: 10.1021/acs.jproteome.5b00703. Epub 2015 Sep 14.
9
Definitive Screening Design Optimization of Mass Spectrometry Parameters for Sensitive Comparison of Filter and Solid Phase Extraction Purified, INLIGHT Plasma N-Glycans.用于灵敏比较滤膜和固相萃取纯化的INLIGHT血浆N-聚糖的质谱参数的确定性筛选设计优化
Anal Chem. 2015 Jul 21;87(14):7305-12. doi: 10.1021/acs.analchem.5b01609. Epub 2015 Jun 30.
10
Conformational changes associated with post-translational modifications of Pro(143) in Skp1 of Dictyostelium--a dipeptide model system.与盘基网柄菌Skp1中Pro(143)的翻译后修饰相关的构象变化——一个二肽模型系统
J Am Chem Soc. 2014 Oct 29;136(43):15170-5. doi: 10.1021/ja5033277. Epub 2014 Oct 16.
本文引用的文献
1
Sequence-Specific Peptide-Carbohydrate Interactions in an Asparagine-Linked Glycopeptide.
J Org Chem. 1996 Jun 26;61(13):4198-4199. doi: 10.1021/jo960590c.
2
Analysis of the conserved glycosylation site in the nicotinic acetylcholine receptor: potential roles in complex assembly.烟碱型乙酰胆碱受体中保守糖基化位点的分析：在复合物组装中的潜在作用
Chem Biol. 1995 Nov;2(11):751-9. doi: 10.1016/1074-5521(95)90103-5.
3
Structural comparison of a 15 residue peptide from the V3 loop of HIV-1IIIb and an O-glycosylated analogue.
FEBS Lett. 1996 Sep 16;393(2-3):280-6. doi: 10.1016/0014-5793(96)00912-x.
4
1H NMR studies on an Asn-linked glycopeptide. GlcNAc-1 C2-N2 bond is rigid in H2O.关于天冬酰胺连接的糖肽的核磁共振氢谱研究。在水中，N-乙酰葡糖胺-1的C2-N2键是刚性的。
J Biol Chem. 1994 Feb 4;269(5):3331-8.
5
Conformational implications of asparagine-linked glycosylation.天冬酰胺连接的糖基化的构象影响。
Proc Natl Acad Sci U S A. 1995 Jan 3;92(1):97-101. doi: 10.1073/pnas.92.1.97.
6
A general approach to the synthesis of O- and N-linked glycopeptides.一种合成O-连接和N-连接糖肽的通用方法。
Glycoconj J. 1994 Apr;11(2):59-63. doi: 10.1007/BF00731144.
7
A strategy for a convergent synthesis of N-linked glycopeptides on a solid support.一种在固相载体上收敛合成N-连接糖肽的策略。
Science. 1995 Jul 14;269(5221):202-4. doi: 10.1126/science.7618080.
8
Conformation and function of the N-linked glycan in the adhesion domain of human CD2.人CD2黏附结构域中N-连接聚糖的构象与功能
Science. 1995 Sep 1;269(5228):1273-8. doi: 10.1126/science.7544493.
9
Acquisition of three-dimensional structure of proteins.蛋白质三维结构的获取。
Annu Rev Biochem. 1973;42:135-58. doi: 10.1146/annurev.bi.42.070173.001031.
10
Folding of immunogenic peptide fragments of proteins in water solution. I. Sequence requirements for the formation of a reverse turn.蛋白质免疫原性肽片段在水溶液中的折叠。I. 形成反向转角的序列要求。
J Mol Biol. 1988 May 5;201(1):161-200. doi: 10.1016/0022-2836(88)90446-9.
Zotero 插件