Imperiali B, O'Connor S E
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Curr Opin Chem Biol. 1999 Dec;3(6):643-9. doi: 10.1016/s1367-5931(99)00021-6.
Asparagine-linked glycosylation is an enzyme-catalyzed, co-translational protein modification reaction that has the capacity to influence either the protein folding process or the stability of the native glycoprotein conjugate. Advances in both glycoconjugate chemical synthesis and glycoprotein expression methods have increased the availability of these once elusive biopolymers. The application of spectroscopic methods to these proteins has begun to illuminate the various ways in which the saccharide affects the structure, function and stability of the proteins.
天冬酰胺连接的糖基化是一种酶催化的共翻译蛋白质修饰反应，它有能力影响蛋白质折叠过程或天然糖蛋白缀合物的稳定性。糖缀合物化学合成和糖蛋白表达方法的进展增加了这些曾经难以获得的生物聚合物的可及性。光谱方法在这些蛋白质上的应用已开始阐明糖类影响蛋白质结构、功能和稳定性的各种方式。
