YY1介导的转录抑制作用是通过与酵母全局调节因子RPD3的哺乳动物同源物相互作用来实现的。
Transcriptional repression by YY1 is mediated by interaction with a mammalian homolog of the yeast global regulator RPD3.
作者信息
Yang W M, Inouye C, Zeng Y, Bearss D, Seto E
机构信息
Moffitt Cancer Center and Research Institute, University of South Florida, Tampa 33612, USA.
出版信息
Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12845-50. doi: 10.1073/pnas.93.23.12845.
Abstract
YY1 is a mammalian zinc-finger transcription factor with unusual structural and functional features. It has been implicated as a positive and a negative regulatory factor that binds to the CCATNTT consensus DNA element located in promoters of many cellular and viral genes. A mammalian cDNA that encodes a YY1-binding protein and possesses sequence homology with the yeast transcriptional factor RPD3 has been identified. A Gal4 DNA binding domain-mammalian RPD3 fusion protein strongly represses transcription from a promoter containing Gal4 binding sites. Association between YY1 and mammalian RPD3 requires a glycine-rich region on YY1. Mutations in this region abolish the interaction with mammalian RPD3 and eliminate transcriptional repression by YY1. These data suggest that YY1 negatively regulates transcription by tethering RPD3 to DNA as a cofactor and that this transcriptional mechanism is highly conserved from yeast to human.
摘要
YY1是一种具有独特结构和功能特征的哺乳动物锌指转录因子。它被认为是一种正负调节因子，可与位于许多细胞和病毒基因启动子中的CCATNTT共有DNA元件结合。已鉴定出一种编码与YY1结合蛋白且与酵母转录因子RPD3具有序列同源性的哺乳动物cDNA。Gal4 DNA结合结构域-哺乳动物RPD3融合蛋白强烈抑制含有Gal4结合位点的启动子的转录。YY1与哺乳动物RPD3之间的关联需要YY1上的富含甘氨酸区域。该区域的突变消除了与哺乳动物RPD3的相互作用，并消除了YY1的转录抑制作用。这些数据表明，YY1通过将RPD3作为辅助因子与DNA相连来负调节转录，并且这种转录机制从酵母到人类高度保守。
相似文献
1
Transcriptional repression by YY1 is mediated by interaction with a mammalian homolog of the yeast global regulator RPD3.YY1介导的转录抑制作用是通过与酵母全局调节因子RPD3的哺乳动物同源物相互作用来实现的。
Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12845-50. doi: 10.1073/pnas.93.23.12845.
2
Biological activity of mammalian transcriptional repressors.哺乳动物转录抑制因子的生物学活性。
Biol Chem. 2001 Jun;382(6):891-902. doi: 10.1515/BC.2001.111.
3
Conservation and divergence of the yeast and mammalian unfolded protein response. Activation of specific mammalian endoplasmic reticulum stress element of the grp78/BiP promoter by yeast Hac1.酵母与哺乳动物未折叠蛋白反应的保守性与差异性。酵母Hac1对grp78/BiP启动子的特定哺乳动物内质网应激元件的激活。
J Biol Chem. 1999 Oct 22;274(43):30402-9. doi: 10.1074/jbc.274.43.30402.
4
Regulation of transcription factor YY1 by acetylation and deacetylation.转录因子YY1的乙酰化与去乙酰化调控
Mol Cell Biol. 2001 Sep;21(17):5979-91. doi: 10.1128/MCB.21.17.5979-5991.2001.
5
Transcriptional repression by YY1, a human GLI-Krüppel-related protein, and relief of repression by adenovirus E1A protein.人源GLI-Krüppel相关蛋白YY1的转录抑制作用以及腺病毒E1A蛋白对抑制作用的解除。
Cell. 1991 Oct 18;67(2):377-88. doi: 10.1016/0092-8674(91)90189-6.
6
Yin yang 1 protein negatively regulates high-density lipoprotein receptor gene transcription by disrupting binding of sterol regulatory element binding protein to the sterol regulatory element.阴阳1蛋白通过破坏固醇调节元件结合蛋白与固醇调节元件的结合来负向调节高密度脂蛋白受体基因转录。
Endocrinology. 2001 Jan;142(1):49-58. doi: 10.1210/endo.142.1.7868.
7
Repression by Ume6 involves recruitment of a complex containing Sin3 corepressor and Rpd3 histone deacetylase to target promoters.Ume6介导的基因沉默作用涉及到一个包含Sin3共抑制因子和Rpd3组蛋白去乙酰化酶的复合物被招募到目标启动子上。
Cell. 1997 May 2;89(3):365-71. doi: 10.1016/s0092-8674(00)80217-2.
8
The yeast alpha2 and Mcm1 proteins interact through a region similar to a motif found in homeodomain proteins of higher eukaryotes.酵母α2蛋白和Mcm1蛋白通过一个与在高等真核生物同源结构域蛋白中发现的模体相似的区域相互作用。
Mol Cell Biol. 1996 May;16(5):2135-43. doi: 10.1128/MCB.16.5.2135.
9
YY1 and NF1 both activate the human p53 promoter by alternatively binding to a composite element, and YY1 and E1A cooperate to amplify p53 promoter activity.YY1和NF1都通过交替结合一个复合元件来激活人类p53启动子，并且YY1和E1A协同作用以增强p53启动子活性。
Mol Cell Biol. 1996 Oct;16(10):5933-45. doi: 10.1128/MCB.16.10.5933.
10
Histone deacetylase activity of Rpd3 is important for transcriptional repression in vivo.Rpd3的组蛋白去乙酰化酶活性对于体内转录抑制很重要。
Genes Dev. 1998 Mar 15;12(6):797-805. doi: 10.1101/gad.12.6.797.
引用本文的文献
1
The FcRn from gene to protein and function: comparison between species.从基因到蛋白质及功能的新生儿Fc受体：物种间比较
Front Immunol. 2025 Aug 1;16:1608426. doi: 10.3389/fimmu.2025.1608426. eCollection 2025.
2
Ectopic USP15 expression inhibits HIV-1 transcription involving changes in YY1 deubiquitination and stability.异位USP15表达通过改变YY1去泛素化和稳定性来抑制HIV-1转录。
Front Cell Infect Microbiol. 2024 Nov 18;14:1371655. doi: 10.3389/fcimb.2024.1371655. eCollection 2024.
3
Unveiling the role of histone deacetylases in neurological diseases: focus on epilepsy.揭示组蛋白去乙酰化酶在神经疾病中的作用：聚焦癫痫
Biomark Res. 2024 Nov 19;12(1):142. doi: 10.1186/s40364-024-00687-6.
4
Molecular docking of curcumin and curcuminoids as human Zn dependent histone deacetylase (HDAC) enzyme inhibitors.姜黄素及姜黄素类化合物作为人锌依赖性组蛋白脱乙酰酶（HDAC）抑制剂的分子对接
In Silico Pharmacol. 2024 May 28;12(1):47. doi: 10.1007/s40203-024-00221-4. eCollection 2024.
5
Domestication Gene and Its Partner Are Involved in Controlling the Larval Body Size and Cocoon Shell Weight of .家蚕驯化基因及其伙伴参与控制幼虫体质量和茧层量。
Int J Mol Sci. 2024 Mar 18;25(6):3427. doi: 10.3390/ijms25063427.
6
Chemical Versatility in Catalysis and Inhibition of the Class IIb Histone Deacetylases.催化和抑制 IIb 类组蛋白去乙酰化酶中的化学多功能性。
Acc Chem Res. 2024 Apr 16;57(8):1135-1148. doi: 10.1021/acs.accounts.3c00801. Epub 2024 Mar 26.
7
Breaking the Silence: Regulation of HIV Transcription and Latency on the Road to a Cure.打破沉默：治愈艾滋病道路上的 HIV 转录和潜伏期调控。
Viruses. 2023 Dec 15;15(12):2435. doi: 10.3390/v15122435.
8
Assessing the dynamics and macromolecular interactions of the intrinsically disordered protein YY1.评估无规则卷曲蛋白 YY1 的动态变化和大分子相互作用。
Biosci Rep. 2023 Oct 31;43(10). doi: 10.1042/BSR20231295.
9
Zinc Ions Modulate YY1 Activity: Relevance in Carcinogenesis.锌离子调节YY1活性：在致癌作用中的相关性。
Cancers (Basel). 2023 Aug 30;15(17):4338. doi: 10.3390/cancers15174338.
10
The Role of Histone Deacetylases in Acute Lung Injury-Friend or Foe.组蛋白去乙酰化酶在急性肺损伤中的作用：是敌是友。
Int J Mol Sci. 2023 Apr 26;24(9):7876. doi: 10.3390/ijms24097876.
本文引用的文献
1
The Yin and the Yang of mammalian transcription.哺乳动物转录的阴阳学说。
Curr Biol. 1992 Mar;2(3):152-4. doi: 10.1016/0960-9822(92)90268-f.
2
A mammalian histone deacetylase related to the yeast transcriptional regulator Rpd3p.一种与酵母转录调节因子Rpd3p相关的哺乳动物组蛋白脱乙酰基酶。
Science. 1996 Apr 19;272(5260):408-11. doi: 10.1126/science.272.5260.408.
3
Characterization of functional domains within the multifunctional transcription factor, YY1.多功能转录因子YY1内功能域的表征
J Biol Chem. 1995 Dec 15;270(50):30213-20. doi: 10.1074/jbc.270.50.30213.
4
The RXR heterodimers and orphan receptors.视黄酸X受体异二聚体和孤儿受体。
Cell. 1995 Dec 15;83(6):841-50. doi: 10.1016/0092-8674(95)90200-7.
5
Evidence for physical interaction between the zinc-finger transcription factors YY1 and Sp1.锌指转录因子YY1和Sp1之间存在物理相互作用的证据。
Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6145-9. doi: 10.1073/pnas.90.13.6145.
6
Transcriptional repression in eukaryotes.真核生物中的转录抑制
Annu Rev Cell Biol. 1993;9:479-509. doi: 10.1146/annurev.cb.09.110193.002403.
7
Inhibition of transcriptional regulator Yin-Yang-1 by association with c-Myc.通过与c-Myc结合抑制转录调节因子阴阳-1
Science. 1993 Dec 17;262(5141):1889-92. doi: 10.1126/science.8266081.
8
Repression versus activation in the control of gene transcription.基因转录调控中的抑制与激活
Trends Biochem Sci. 1994 Jan;19(1):38-42. doi: 10.1016/0968-0004(94)90172-4.
9
TATA-binding protein-independent initiation: YY1, TFIIB, and RNA polymerase II direct basal transcription on supercoiled template DNA.不依赖TATA结合蛋白的起始：YY1、TFIIB和RNA聚合酶II在超螺旋模板DNA上指导基础转录。
Cell. 1994 Mar 25;76(6):1115-21. doi: 10.1016/0092-8674(94)90387-5.
10
Relief of YY1-induced transcriptional repression by protein-protein interaction with the nucleolar phosphoprotein B23.通过与核仁磷蛋白B23的蛋白质-蛋白质相互作用缓解YY1诱导的转录抑制。
J Biol Chem. 1994 Mar 4;269(9):6506-10.
Zotero 插件