小鼠CXB重组近交系中运动活动昼夜节律周期的假设数量性状基因座（QTL）

Hypothetical quantitative trait loci (QTL) for circadian period of locomotor activity in CXB recombinant inbred strains of mice.

作者信息

Mayeda A R, Hofstetter J R, Belknap J K, Nurnberger J I

机构信息

Department of Psychiatry, Indiana University, Indianapolis 46202, USA.

出版信息

Behav Genet. 1996 Sep;26(5):505-11. doi: 10.1007/BF02359755.

DOI:10.1007/BF02359755

PMID:8917949

Abstract

The locomotor activity of male mice (Mus musculus) of 13 CXB (BALB/cBy x C57BL/6J) recombinant inbred (RI) strains and their progenitor strains was monitored for 4 to 6 weeks by infrared photoelectric beams under constant dark. The circadian period (tau) of locomotor activity was calculated and used in quantitative trait locus (QTL) analysis of strains' means. Results were compared with potential QTL found in a previous study of the BXD RI series. The mean tau of 13 CXB RI mouse strains (three to six animals per strain) in constant dark had a unimodal distribution suggesting polygenic inheritance. A number of potential QTL were found for this trait. There were two associations at p < .001. H23 on chromosome 3 and Pmv16 on chromosome 16. A region of chromosome 1 was associated with tau in both CXB and BXD RI series. There was also a conjunction with a locus determined from QTL analysis of the previously reported tau of wheel running activity in seven CXB RI strains (Schwartz and Zimmerman, 1990).

摘要

在持续黑暗条件下，通过红外光电束对13个CXB（BALB/cBy×C57BL/6J）重组近交（RI）品系的雄性小鼠（小家鼠）及其亲本品系进行了4至6周的运动活动监测。计算了运动活动的昼夜节律周期（tau），并将其用于品系均值的数量性状基因座（QTL）分析。将结果与先前对BXD RI系列研究中发现的潜在QTL进行了比较。在持续黑暗中，13个CXB RI小鼠品系（每个品系3至6只动物）的平均tau呈单峰分布，表明存在多基因遗传。发现了该性状的一些潜在QTL。在p < 0.001水平上有两个关联，分别是3号染色体上的H23和16号染色体上的Pmv16。1号染色体的一个区域在CXB和BXD RI系列中均与tau相关。此外，还与先前报道的7个CXB RI品系的转轮运动活动tau的QTL分析确定的一个基因座存在关联（施瓦茨和齐默尔曼，1990年）。