Ebenfelt A, Lundqvist H, Dahlgren C, Lundberg C
Department of Otorhinolaryngology, Head and Neck Surgery, Sahlgrenska University Hospital, Göteborg, Sweden.
Clin Exp Immunol. 1996 Nov;106(2):404-9. doi: 10.1046/j.1365-2249.1996.d01-846.x.
The leucocytes in the secretion on the tonsillar surface have been regarded as inactivated and dying cells with no essential function in the defence of the underlying mucosa. In recent studies, we showed that in acute tonsillitis there are very few, if any, bacteria in the parenchyma, whereas in the secretion on the tonsillar surface there are huge numbers of bacteria and neutrophils, and furthermore, extensive phagocytosis. The present study was performed to elucidate the functional capacity of the neutrophils in the secretion on the tonsillar surface. Secretion samples were taken from the tonsillar surface of healthy volunteers with an imprint technique which allows transfer of secretion from the mucosa to a glass slide with maintained topographic position of the cells. The capacity of the neutrophils to respond to chemotactic stimuli and to phagocytize and further process labelled yeast particles was studied in this in vitro system. The results show that the neutrophils in the secretion have the same properties as in tissue, and also when having arrived in the secretion they can identify and attack new prey.
扁桃体表面分泌物中的白细胞一直被认为是失活和即将死亡的细胞，在保护其下方的黏膜方面没有重要功能。在最近的研究中，我们发现，在急性扁桃体炎时，实质中几乎没有细菌（即便有也极少），而扁桃体表面的分泌物中有大量细菌和中性粒细胞，而且存在广泛的吞噬作用。本研究旨在阐明扁桃体表面分泌物中中性粒细胞的功能能力。采用印记技术从健康志愿者的扁桃体表面采集分泌物样本，该技术可将分泌物从黏膜转移至载玻片上，同时保持细胞的拓扑位置。在这个体外系统中，研究了中性粒细胞对趋化刺激的反应能力、吞噬并进一步处理标记酵母颗粒的能力。结果表明，分泌物中的中性粒细胞与组织中的中性粒细胞具有相同的特性，并且当它们进入分泌物后，仍能识别并攻击新的猎物。
