PSO4和PRP19的等位性将酿酒酵母中的前体mRNA加工与重组及易错DNA修复联系起来。
Allelism of PSO4 and PRP19 links pre-mRNA processing with recombination and error-prone DNA repair in Saccharomyces cerevisiae.
作者信息
Grey M, Düsterhöft A, Henriques J A, Brendel M
机构信息
Institut für Mikrobiologie der J.W.Goethe Universität, Frankfurt/Main,Germany.
出版信息
Nucleic Acids Res. 1996 Oct 15;24(20):4009-14. doi: 10.1093/nar/24.20.4009.
Abstract
The radiation-sensitive mutant pso4-1 of Saccharomyces cerevisiae shows a pleiotropic phenotype, including sensitivity to DNA cross-linking agents, nearly blocked sporulation and reduced mutability. We have cloned the putative yeast DNA repair gene PSO4 from a genomic library by complementation of the blocked UV-induced mutagenesis and of sporulation in diploids homozygous for pso4-1. Sequence analysis revealed that gene PSO4 consists of 1512 bp located upstream of UBI4 on chromosome XII and encodes a putative protein of 56.7 kDa. PSO4 is allelic to PRP19, a gene encoding a spliceosome-associated protein, but shares no significant homology with other yeast genes. Gene disruption with a destroyed reading frame of our PSO4 clone resulted in death of haploid cells, confirming the finding that PSO4/PRP19 is an essential gene. Thus, PSO4 is the third essential DNA repair gene found in the yeast S.cerevisiae.
摘要
酿酒酵母的辐射敏感突变体pso4-1表现出多效性表型，包括对DNA交联剂敏感、孢子形成几乎受阻以及突变率降低。我们通过对pso4-1纯合二倍体中紫外线诱导的诱变受阻和孢子形成进行互补，从基因组文库中克隆了推定的酵母DNA修复基因PSO4。序列分析表明，基因PSO4由位于第十二号染色体上UBI4上游的1512 bp组成，编码一种推定的56.7 kDa蛋白质。PSO4与PRP19等位，PRP19是一个编码剪接体相关蛋白的基因，但与其他酵母基因没有明显的同源性。用我们的PSO4克隆的破坏阅读框进行基因破坏导致单倍体细胞死亡，证实了PSO4/PRP19是一个必需基因的发现。因此，PSO4是在酿酒酵母中发现的第三个必需DNA修复基因。
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本文引用的文献
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Yeast nucleotide excision repair proteins Rad2 and Rad4 interact with RNA polymerase II basal transcription factor b (TFIIH).酵母核苷酸切除修复蛋白Rad2和Rad4与RNA聚合酶II基础转录因子b（TFIIH）相互作用。
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