Eldadah B A, Yakovlev A G, Faden A I
Department of Neurology and the Georgetown Institute for Cognitive and Computational Sciences, Georgetown University Medical Center, Washington, DC 20007, USA.
Nucleic Acids Res. 1996 Oct 15;24(20):4092-3. doi: 10.1093/nar/24.20.4092.
Apoptotic cell death is often characterized by internucleosomal cleavage of genomic DNA, which exhibits a distinctive ladder upon electrophoresis. However, techniques used for the isolation and detection of DNA to demonstrate laddering may not be sufficiently sensitive, particularly when cleaved DNA is present at modest levels. We propose a new approach for isolating total cellular DNA using a silica-based resin that improves the resolution of DNA laddering. In addition, we introduce a rapid DNA labeling method that can increase the sensitivity of detecting DNA laddering. Each of these methods can be used for DNA from cell cultures or tissues.
凋亡性细胞死亡通常以基因组DNA的核小体间切割为特征，这种切割在电泳时呈现出独特的梯状条带。然而，用于分离和检测DNA以显示梯状条带的技术可能不够灵敏，特别是当切割后的DNA含量适中时。我们提出了一种使用基于硅胶的树脂分离总细胞DNA的新方法，该方法提高了DNA梯状条带的分辨率。此外，我们引入了一种快速DNA标记方法，可提高检测DNA梯状条带的灵敏度。这些方法均可用于从细胞培养物或组织中提取的DNA。
