Iyngkaran N, Yadav M, Boey C G
Department of Paediatrics, University Hospital, Malaysia.
Singapore Med J. 1995 Aug;36(4):393-6.
Enterokinase has a critical role in initiating proteolytic digestion by hydrolysing the conversion of pancreatic trypsinogen into trypsin. The enzyme is synthesised by enterocytes of the proximal small intestine and initially incorporated into the brush border from where it is released into the intestinal lumen by the action of pancreatic secretions. The aim of the study was to analyse enterokinase activity in the duodenal mucosa of infants with diarrhoeal disease including cow's milk protein-sensitive enteropathy. Our observations show that the mean depletion of enterokinase was only 17% compared to 60-80% for other brush border enzymes like disaccharidases, peptidases and alkaline phosphatases in infants with diarrhoea. This suggests that enterokinase activity in the small bowel enteropathies may be dependent not only on the degree of mucosal damage specifically but also on the extent of damage to the goblet cell population where the enzyme is synthesised. Thus the enterokinase activity was reduced in acute and chronic diarrhoea with marked mucosal damage where significant reduction of goblet cell population was evident but the enzyme was relatively little affected when the mucosa was damaged mildly.
肠激酶通过水解将胰蛋白酶原转化为胰蛋白酶，在启动蛋白水解消化过程中起关键作用。该酶由近端小肠的肠上皮细胞合成，最初整合到刷状缘，在胰腺分泌物的作用下从刷状缘释放到肠腔中。本研究的目的是分析患有腹泻病（包括牛乳蛋白敏感性肠病）的婴儿十二指肠黏膜中的肠激酶活性。我们的观察结果表明，与腹泻婴儿中其他刷状缘酶（如双糖酶、肽酶和碱性磷酸酶）60%-80%的减少相比，肠激酶的平均减少仅为17%。这表明小肠肠病中的肠激酶活性不仅可能特别取决于黏膜损伤的程度，还取决于合成该酶的杯状细胞群体的损伤程度。因此，在急性和慢性腹泻伴明显黏膜损伤且杯状细胞群体明显减少时，肠激酶活性降低，但当黏膜轻度受损时，该酶受影响相对较小。
