Garrido J J, de Andres D F, Pintado C O, Llanes D, Stear M J
Departmento de Genetica, Facultad de Veterinaria, Universidad de Cordoba, Espana.
Exp Clin Immunogenet. 1995;12(4):268-71.
Using segregation and population analysis of serological data prepared in our laboratory, we have tentatively defined five OLA antigenic specificities: CO1.1, CO1.2, CO2.1 and CO2.2, whose genetic control would fit a model of two class I OLA loci (CO1.1 and CO1.2 first locus, CO2.1 and CO2.2 second locus, and CO3.1, that could represent a subtype of CO2.2. Alloantisera were obtained from Spanish Merino sheep by intrafamilial cross-immunization and from serum of primiparous and multiparous ewes. Screening of alloantisera for antilymphocyte reactivity was carried out by a two-step dye exclusion microcytotoxicity test against 216 animals belonging to different local breeds: Spanish Merino, Merino Landschaft, Manchega and Churra. Lymphocyte antigen specificities were characterized by cluster and correlation analysis, as well as family studies. Comparison of CO alloantisera with reference reagents from Scotland (CA reagents) showed an excellent correlation between CO1.1 and CA2, and CO2.1 and CA5. The reagents described can be used in various breeds, a characteristic important for their future applications.
利用我们实验室所制备血清学数据的分离分析和群体分析，我们初步确定了5种OLA抗原特异性：CO1.1、CO1.2、CO2.1和CO2.2，其遗传控制符合两个I类OLA位点的模型（CO1.1和CO1.2为第一个位点，CO2.1和CO2.2为第二个位点，而CO3.1可能代表CO2.2的一个亚型）。同种异体抗血清是通过家族内交叉免疫从西班牙美利奴羊获得的，也来自初产和经产母羊的血清。通过两步染料排除微细胞毒性试验，对属于不同本地品种的216只动物（西班牙美利奴羊、美利奴兰茨查夫羊、曼彻加羊和丘拉羊）进行抗淋巴细胞反应性的同种异体抗血清筛选。淋巴细胞抗原特异性通过聚类分析、相关性分析以及家系研究来表征。将CO同种异体抗血清与来自苏格兰的参考试剂（CA试剂）进行比较，结果显示CO1.1与CA2、CO2.1与CA5之间具有良好的相关性。所描述的试剂可用于各种品种，这一特性对其未来应用很重要。
