基于肝螺杆菌16S rRNA基因核苷酸序列的诊断检测方法。
Diagnostic assay for Helicobacter hepaticus based on nucleotide sequence of its 16S rRNA gene.
作者信息
Battles J K, Williamson J C, Pike K M, Gorelick P L, Ward J M, Gonda M A
机构信息
Laboratory of Cell and Molecular Structure, Program Resources, Inc./DynCorp, National Cancer Institute-Frederick Cancer Research and Development Center, Maryland 21702-1201, USA.
出版信息
J Clin Microbiol. 1995 May;33(5):1344-7. doi: 10.1128/jcm.33.5.1344-1347.1995.
Abstract
Conserved primers were used to PCR amplify 95% of the Helicobacter hepaticus 16S rRNA gene. Its sequence was determined and aligned to those of related bacteria, enabling the selection of primers to highly diverged regions of the 16S rRNA gene and an oligonucleotide probe for the development of a PCR-liquid hybridization assay. This assay was shown to be both sensitive and specific for H. hepaticus 16S rRNA gene sequences.
摘要
使用保守引物通过聚合酶链反应（PCR）扩增肝螺杆菌16S rRNA基因的95%。测定其序列并与相关细菌的序列进行比对，从而能够选择针对16S rRNA基因高度分化区域的引物以及用于开发PCR-液相杂交检测的寡核苷酸探针。该检测方法对肝螺杆菌16S rRNA基因序列显示出灵敏性和特异性。
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