Satiat-Jeunemaitre B, Cole L, Bourett T, Howard R, Hawes C
Institut des Sciences Végétales, C.N.R.S., UPR40, Gif/Yvette, France.
J Microsc. 1996 Feb;181(Pt 2):162-77. doi: 10.1046/j.1365-2818.1996.112393.x.
Whilst the function and organization of the secretory machinery in eukaryotic cells exhibit basic similarities, the compartmentation of the endomembrane system can show significant differences between the fungal, plant and animal kingdoms. The use of the antibiotic brefeldin A (BFA) as an inhibitor of secretion in both animal and yeast cells has resulted in a remarkable advance in our understanding of the modes of action of vesicle shuttles between the endoplasmic reticulum and Golgi apparatus and within the Golgi apparatus itself. It is now apparent that application of the drug to filamentous fungi and plants will also help unravel the workings of the secretory system in these organisms. In this paper we review recent progress in our laboratories on elucidating the effects of BFA on the morphology of the Golgi apparatus and compare these with recently published data on fungal and plant cells. Variation in the response to BFA are reported, which may not all be attributed to differences in drug concentration and time of treatment. These may reflect differences in cellular sensitivity or multiple sites of action of the drug, and the existence of a specific molecular target for BFA is questioned.
虽然真核细胞中分泌机制的功能和组织表现出基本的相似性，但内膜系统的区室化在真菌、植物和动物界之间可能存在显著差异。抗生素布雷菲德菌素A（BFA）作为动物和酵母细胞中分泌的抑制剂，极大地推动了我们对内质网和高尔基体之间以及高尔基体内部囊泡穿梭作用模式的理解。现在很明显，将该药物应用于丝状真菌和植物也将有助于揭示这些生物体中分泌系统的运作方式。在本文中，我们回顾了我们实验室在阐明BFA对高尔基体形态影响方面的最新进展，并将其与最近发表的关于真菌和植物细胞的数据进行比较。报道了对BFA反应的差异，这可能并非都归因于药物浓度和处理时间的差异。这些差异可能反映了细胞敏感性的差异或药物的多个作用位点，并且有人质疑BFA是否存在特定的分子靶点。
