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在面包酵母的结构化模型中,从乙醇氧化到葡萄糖利用的转化率动力学

Transition rate kinetics from ethanol oxidation to glucose utilisation within a structured model of baker's yeast.

作者信息

Dantigny P, Gruber M

机构信息

Laboratoire de microbiologie appliquée et industrielle, Université Claude Bernard, Lyon, France.

出版信息

Appl Microbiol Biotechnol. 1996 Mar;45(1-2):199-203. doi: 10.1007/s002530050670.

Abstract

The transition rate kinetics from ethanol oxidation to glucose utilisation, within a structured model of baker's yeast, described previously, were experimentally identified. The shift in metabolism has been assessed through glucose pulses during batch growth on ethanol. The influence of glucose concentration (between 0.25 g l-1 and 0.90 g l-1) and initial biomass concentration (between 0.61 g l-1 and 1.44 g l-1) on the transition rate was determined. The transition rate can not be described by a first-order saturation-type kinetics with respect to glucose only. A corrective term, which takes into account biomass concentration should be included.

摘要

在先前描述的面包酵母结构化模型中,对从乙醇氧化到葡萄糖利用的转化率动力学进行了实验鉴定。通过在乙醇分批培养过程中进行葡萄糖脉冲实验,评估了代谢转变情况。测定了葡萄糖浓度(在0.25 g l-1至0.90 g l-1之间)和初始生物量浓度(在0.61 g l-1至1.44 g l-1之间)对转化率的影响。转化率不能仅用关于葡萄糖的一级饱和型动力学来描述。应纳入一个考虑生物量浓度的校正项。

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