Teifke J P, Löhr C V
Institut für Veterinär-Pathologie, Justus-Liebig-Universität, Giessen, Germany.
J Comp Pathol. 1996 Feb;114(2):205-10. doi: 10.1016/s0021-9975(96)80010-7.
One hundred and six squamous cell carcinomas (SCCs) of cattle, horses, cats and dogs were analysed immunohistochemically for overexpression of p53 protein. The monoclonal antibody pAb 240, which recognizes only mutant p53, was used. Of 41 bovine ocular SCCs, 26 (63.4%) showed p53 nuclear reactivity. All of six (100%) equine ocular SCCs and seven of nine (77.7%) SCCs of the equine penis or vulva gave positive reactions. In nine of 11 (81.8%) feline SCCs of the ear and in seven of 14 (50%) feline SCCs of other locations, p53 immunoreactivity was detected. Only seven of 25 (29.5%) canine cutaneous SCCs gave a positive reaction. Thus p53 antigen could be detected immunohistochemically in formalin-fixed and paraffin wax-embedded tissues of SCCs of domestic animals. The results support the view that, as in man, p53 overexpression plays an important role in the development of most SCCs of the animal species studied. This was in particular true for feline SCCs of the ear and for bovine and equine ocular SCCs, which are assumed to be related to ultraviolet radiation.
对106例牛、马、猫和狗的鳞状细胞癌（SCC）进行免疫组织化学分析，以检测p53蛋白的过表达情况。使用了仅识别突变型p53的单克隆抗体pAb 240。在41例牛眼SCC中，26例（63.4%）显示p53核反应阳性。6例（100%）马眼SCC以及9例马阴茎或外阴SCC中的7例（77.7%）呈阳性反应。在11例猫耳部SCC中的9例（81.8%）以及其他部位的14例猫SCC中的7例（50%）检测到p53免疫反应性。25例犬皮肤SCC中仅7例（29.5%）呈阳性反应。因此，在家畜SCC的福尔马林固定石蜡包埋组织中可通过免疫组织化学检测到p53抗原。结果支持这样一种观点，即与人类一样，p53过表达在大多数所研究动物物种的SCC发生过程中起重要作用。这在耳部猫SCC以及牛和马眼SCC中尤其如此，这些被认为与紫外线辐射有关。
