Spieker R L
Max-Planck-Institut für Biochemie, Abteilung Viroidforschung, Martinsried, Federal Republic of Germany.
Arch Virol. 1996;141(10):1823-32. doi: 10.1007/BF01718197.
A viroid was isolated from symptomless Brunfelsia undulata plants using the bidirectional PAGE method for analysis of small circular RNA molecules. The viroid was transmitted to tomato by mechanical inoculation. Infected tomato plants developed symptoms similar to those caused by intermediate strains of potato spindle tuber viroid (PSTVd). Cloning and sequencing revealed that the viroid from Brunfelsia undulata is closely related to the Columnea latent viroid (CLVd). Therefore, the new viroid sequence variant has been named CLVd-B. The Brunfelsia viroid CLVd-B consists of 373 nucleotides, 215 G + C, 158 A + U with a GC content of 57.6%. The most stable rod-like secondary structure of this viroid has 80 G:C, 39 A:U and 11 G:U base pairs with a minimum free energy of -157.2 kcal/mol (-657.1 kJ/mol). The sequence similarity of the right terminal domain of CLVd-B and of tomato apical stunt viroid (TASVd) is higher than the sequence similarity of these domains comparing CLVd and TASVd.
采用双向聚丙烯酰胺凝胶电泳法分析小环状RNA分子，从无症状的波叶布伦felsia植株中分离出一种类病毒。该类病毒通过机械接种传播到番茄上。受感染的番茄植株出现了与马铃薯纺锤块茎类病毒（PSTVd）中间株系引起的症状相似的症状。克隆和测序结果表明，来自波叶布伦felsia的类病毒与哥伦比亚潜隐类病毒（CLVd）密切相关。因此，这个新的类病毒序列变体被命名为CLVd-B。波叶布伦felsia类病毒CLVd-B由373个核苷酸组成，其中G + C含量为215，A + U含量为158，GC含量为57.6%。该类病毒最稳定的棒状二级结构有80个G:C、39个A:U和11个G:U碱基对，最小自由能为-157.2千卡/摩尔（-657.1千焦/摩尔）。CLVd-B右末端结构域与番茄顶端矮化类病毒（TASVd）的序列相似性高于CLVd和TASVd这些结构域的序列相似性。
