Adám E, Nász I, Hudecz F, Lengyel A, Mezö G, Dobay O
Institute of Microbiology, Semmelweis Medical University, Budapest, Hungary.
Arch Virol. 1998;143(9):1669-82. doi: 10.1007/s007050050408.
The specification and differentiation of eighteen intertype specific (IT) epitopes of adenovirus hexons defined by monoclonal antibodies are given by two groups of hexon types: on which they are and on which they are not present. The close specificity relationship among some groups of epitopes determined by pairwise analysis according to their presence on the hexon types indicate that they could be continuous (sequential), overlapping or discontinuous (topographic) epitopes. Based on the identification of the hydrophilic regions and the localization of beta-turns sixteen IT epitope sites were predicted on human adenovirus (HAdV) type 2 and nineteen on HAdV-41 hexon's amino acid (aa) sequences beside the type and genus specific ones. The 16 predicted IT epitopes on HAdV-2 hexon show good coincidency with the 14 IT epitopes demonstrated with monoclonal antibodies (MAbs) on this hexon type. The predicted number of common epitopes between HAdV types 2 and 41 also corresponds well with the eight IT epitopes determined by MAbs, but the 17 predicted non-common epitopes indicate the possibility of the existence of more epitopes on these hexon types. The location of the predicted epitopes of HAdV-2 were determined by alignment of their sequence numbers on the three dimensional ribbon representation of the hexon subunit. Most of the predicted IT epitopes were found between the type and genus specific epitopes i.e. in the "upper" regions of pedestal domains P1 and P2 orientated toward the virion surface and in the "lower" part of loop 1 region orientated inside the virion. Two peptides representing potential IT epitopes were synthesized corresponding to residues 309-320 from the "lower" part of loop 1 and 399-408 from the "upper" part of P1 region of HAdV-2 hexon. Antibodies raised against the peptide-carrier conjugates recognized different purified native hexon types in ELISA.
由单克隆抗体定义的腺病毒六邻体的18种型间特异性（IT）表位的特异性和分化由两组六邻体类型给出：它们存在于哪些六邻体类型以及不存在于哪些六邻体类型。根据表位在六邻体类型上的存在情况通过成对分析确定的一些表位组之间的紧密特异性关系表明，它们可能是连续（顺序）、重叠或不连续（拓扑）表位。基于亲水区的鉴定和β-转角的定位，除了型和属特异性表位外，在人腺病毒2型六邻体的氨基酸（aa）序列上预测了16个IT表位位点，在腺病毒41型六邻体上预测了19个。腺病毒2型六邻体上预测的16个IT表位与该六邻体类型上用单克隆抗体（MAbs）证实的14个IT表位显示出良好的一致性。腺病毒2型和41型之间预测的共同表位数量也与MAbs确定的8个IT表位很好地对应，但17个预测的非共同表位表明这些六邻体类型上可能存在更多表位。腺病毒2型预测表位的位置通过其序列号在六邻体亚基的三维带状表示上的比对来确定。大多数预测的IT表位位于型和属特异性表位之间，即在朝向病毒体表面的基座结构域P1和P2的“上部”区域以及朝向病毒体内部的环1区域的“下部”。合成了代表潜在IT表位的两种肽，分别对应于腺病毒2型六邻体环1“下部”的残基309 - 320和P1区域“上部”的残基399 - 408。针对肽 - 载体缀合物产生的抗体在ELISA中识别不同纯化的天然六邻体类型。
