膜免疫球蛋白μ激活前B细胞转化需要替代或传统轻链。
Surrogate or conventional light chains are required for membrane immunoglobulin mu to activate the precursor B cell transition.
作者信息
Papavasiliou F, Jankovic M, Nussenzweig M C
机构信息
Laboratory of Molecular Immunology, Howard Hughes Medical Institute, Rockefeller University, New York 10021, USA.
出版信息
J Exp Med. 1996 Nov 1;184(5):2025-30. doi: 10.1084/jem.184.5.2025.
Abstract
To examine the role of light chains in early B cell development we combined RAG-1 and lambda 5 mutations to produce mice that expressed neither conventional nor surrogate light chains (RAG-1-/-, lambda 5-/-). Unique heavy and light chain genes were then introduced into the double and single mutant backgrounds. Membrane immunoglobulin (Ig)mu (mIg mu) associated with Ig alpha-Ig beta but was unable to activate the pre-B cell transition in RAG-1-/-lambda 5-/- mice. Either lambda 5 or kappa light chains were sufficient to complement this deficiency. Therefore light chains are absolutely required for a functional Ig signaling module in early B cell development. Our data provide direct evidence for the existence of two pathways for induction of early B cell development: one which is activated through surrogate light chains and mIg mu, and an alternative pathway which uses conventional light chains and mIg mu.
摘要
为了研究轻链在早期B细胞发育中的作用，我们将RAG-1和λ5突变相结合，以产生既不表达传统轻链也不表达替代轻链的小鼠（RAG-1-/-，λ5-/-）。然后将独特的重链和轻链基因导入双突变和单突变背景中。膜免疫球蛋白（Ig）μ（mIgμ）与Igα-Igβ相关，但无法激活RAG-1-/-λ5-/-小鼠中的前B细胞转变。λ5或κ轻链中的任何一种都足以弥补这一缺陷。因此，轻链是早期B细胞发育中功能性Ig信号模块所绝对必需的。我们的数据为早期B细胞发育诱导的两条途径的存在提供了直接证据：一条通过替代轻链和mIgμ激活，另一条替代途径使用传统轻链和mIgμ。
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