Owman C, Blay P, Nilsson C, Lolait S J
Department of Physiology and Neuroscience, Wallenberg Neuroscience Center, University of Lund, Sweden.
Biochem Biophys Res Commun. 1996 Nov 12;228(2):285-92. doi: 10.1006/bbrc.1996.1654.
Using PCR with degenerate primers and screening of a human B-cell lymphoblast cDNA library, a full-length cDNA encoding a 375-amino-acid protein was isolated. It contains seven regions of hydrophobic amino acids probably representing membrane-spanning domains of a novel heptahelix receptor, tentatively named CMKRL2. It shows nearly 30% overall identity with the high-affinity IL8 receptor and similar degree of homology with other chemoattractant receptors, including the "fusin" coreceptors for HIV1. Measurements of various transduction pathways following application of a panel of chemokines to transfected cells failed to evoke any reproducible response. Although the natural ligand for CMKRL2 could, thus, not be identified, receptor expression in spleen and lymph nodes as well as in Burkitt's lymphoma (irrespective of EBV status) supports a functional role in activated B-cells. Receptor message was ubiquitously distributed in normal peripheral tissues and CNS, suggesting that CMKRL2 is expressed in widespread cell populations, such as macrophages and neuroglia.
利用简并引物进行聚合酶链反应（PCR）并筛选人B细胞淋巴母细胞cDNA文库，分离出了一个编码375个氨基酸的蛋白质的全长cDNA。它包含七个疏水氨基酸区域，可能代表一种新型七螺旋受体的跨膜结构域，暂命名为CMKRL2。它与高亲和力白细胞介素8受体的总体一致性接近30%，与其他趋化因子受体的同源程度相似，包括HIV-1的“融合素”共受体。将一组趋化因子应用于转染细胞后，对各种转导途径的测量未能引发任何可重复的反应。因此，虽然无法确定CMKRL2的天然配体，但在脾脏、淋巴结以及伯基特淋巴瘤（无论EBV状态如何）中的受体表达支持其在活化B细胞中的功能作用。受体信息在正常外周组织和中枢神经系统中广泛分布，这表明CMKRL2在广泛的细胞群体中表达，如巨噬细胞和神经胶质细胞。
