三个新基因GPR9、GPR10和GPR14的克隆及染色体定位，这三个基因编码与白细胞介素8、神经肽Y和生长抑素受体相关的受体。

Cloning and chromosomal mapping of three novel genes, GPR9, GPR10, and GPR14, encoding receptors related to interleukin 8, neuropeptide Y, and somatostatin receptors.

作者信息

Marchese A, Heiber M, Nguyen T, Heng H H, Saldivia V R, Cheng R, Murphy P M, Tsui L C, Shi X, Gregor P

机构信息

Addiction Research Foundation, Toronto, Ontario, Canada.

出版信息

Genomics. 1995 Sep 20;29(2):335-44. doi: 10.1006/geno.1995.9996.

DOI:10.1006/geno.1995.9996

PMID:8666380

Abstract

We employed the polymerase chain reaction and genomic DNA library screening to clone novel human genes, GPR9 and GPR10, and a rat gene, GPR14. GPR9, GPR10, and GPR14 each encode G protein-coupled receptors. GPR10 and GPR14 are intronless within their coding regions, while GPR9 contains at least one intron. The receptor encoded by GPR9 shares the highest identity with human IL-8 receptor type B (38% overall and 53% in the transmembrane regions), followed by IL-8 receptor type A (36% overall and 51% in the transmembrane domains). GPR10 encodes a receptor that shares highest identity with the neuropeptide Y receptor (31% overall and 46% in the transmembrane domains). The receptor encoded by GPR14 shares highest identity with the somatostatin receptor SSTR 4 (27% overall and 41% in the transmembrane domains). Fluorescence in situ hybridization analysis localized GPR9 to chromosome 8p11.2-p12 and GPR10 to chromosome 10q25.3-q26.

摘要

我们采用聚合酶链反应和基因组DNA文库筛选技术克隆了新的人类基因GPR9和GPR10，以及一个大鼠基因GPR14。GPR9、GPR10和GPR14各自编码G蛋白偶联受体。GPR10和GPR14在其编码区域内无内含子，而GPR9至少含有一个内含子。GPR9编码的受体与人类B型白细胞介素8受体具有最高的同源性（总体同源性为38%，跨膜区域为53%），其次是A型白细胞介素8受体（总体同源性为36%，跨膜区域为51%）。GPR10编码的受体与神经肽Y受体具有最高的同源性（总体同源性为31%，跨膜区域为46%）。GPR14编码的受体与生长抑素受体SSTR 4具有最高的同源性（总体同源性为27%，跨膜区域为41%）。荧光原位杂交分析将GPR9定位到8号染色体p11.2 - p12区域，将GPR10定位到10号染色体q25.3 - q26区域。