Marchese A, Heiber M, Nguyen T, Heng H H, Saldivia V R, Cheng R, Murphy P M, Tsui L C, Shi X, Gregor P
Addiction Research Foundation, Toronto, Ontario, Canada.
Genomics. 1995 Sep 20;29(2):335-44. doi: 10.1006/geno.1995.9996.
We employed the polymerase chain reaction and genomic DNA library screening to clone novel human genes, GPR9 and GPR10, and a rat gene, GPR14. GPR9, GPR10, and GPR14 each encode G protein-coupled receptors. GPR10 and GPR14 are intronless within their coding regions, while GPR9 contains at least one intron. The receptor encoded by GPR9 shares the highest identity with human IL-8 receptor type B (38% overall and 53% in the transmembrane regions), followed by IL-8 receptor type A (36% overall and 51% in the transmembrane domains). GPR10 encodes a receptor that shares highest identity with the neuropeptide Y receptor (31% overall and 46% in the transmembrane domains). The receptor encoded by GPR14 shares highest identity with the somatostatin receptor SSTR 4 (27% overall and 41% in the transmembrane domains). Fluorescence in situ hybridization analysis localized GPR9 to chromosome 8p11.2-p12 and GPR10 to chromosome 10q25.3-q26.
我们采用聚合酶链反应和基因组DNA文库筛选技术克隆了新的人类基因GPR9和GPR10，以及一个大鼠基因GPR14。GPR9、GPR10和GPR14各自编码G蛋白偶联受体。GPR10和GPR14在其编码区域内无内含子，而GPR9至少含有一个内含子。GPR9编码的受体与人类B型白细胞介素8受体具有最高的同源性（总体同源性为38%，跨膜区域为53%），其次是A型白细胞介素8受体（总体同源性为36%，跨膜区域为51%）。GPR10编码的受体与神经肽Y受体具有最高的同源性（总体同源性为31%，跨膜区域为46%）。GPR14编码的受体与生长抑素受体SSTR 4具有最高的同源性（总体同源性为27%，跨膜区域为41%）。荧光原位杂交分析将GPR9定位到8号染色体p11.2 - p12区域，将GPR10定位到10号染色体q25.3 - q26区域。
