Turner K, Lenton E A
Sheffield Fertility Centre, UK.
Hum Reprod. 1996 Sep;11(9):1966-74. doi: 10.1093/oxfordjournals.humrep.a019526.
Co-culture of human embryos with cell layers has generally shown that blastocyst formation rates are improved compared to routine culture in medium alone. In order to assess this further, we have additionally classified resulting blastocysts according to their morphology and secretion of human chorionic gonadotrophin (HCG). A total of 70 supernumerary human embryos from 15 patients were divided equally and randomly between two culture conditions: (i) co-culture with Vero cells; and (ii) culture in our routine medium. Embryo development and morphology were recorded for up to 14 days in culture. The results showed that embryos on Vero cells had a significantly higher blastocyst formation rate (P < 0.02) by or on day 6 of development than those in routine culture medium alone (77 and 46% respectively). For HCG analysis, the culture medium was changed in both culture systems on days 5, 7, 9, 12 and 14 of embryo development and analysed. Most embryos began to produce HCG between days 7 and 9, with HCG secretion being significantly higher from embryos on Vero cells between days 9 and 12 than from embryos in routine culture (P < 0.03). The morphology of the blastocysts obtained was related to their ability to hatch and produce HCG but was not significantly better for one type of culture system than for the other.
将人类胚胎与细胞层进行共培养，总体结果显示，与单纯在培养基中进行常规培养相比，囊胚形成率有所提高。为了进一步评估这一点，我们还根据囊胚的形态和人绒毛膜促性腺激素（HCG）的分泌情况对所得囊胚进行了分类。来自15名患者的总共70个多余人类胚胎被平均且随机地分配到两种培养条件下：（i）与Vero细胞共培养；（ii）在我们的常规培养基中培养。记录胚胎在培养长达14天的发育情况和形态。结果显示，在发育第6天或之前，与Vero细胞共培养的胚胎的囊胚形成率显著高于单纯在常规培养基中培养的胚胎（分别为77%和46%，P < 0.02）。对于HCG分析，在胚胎发育的第5、7、9、12和14天，在两种培养系统中都更换培养基并进行分析。大多数胚胎在第7天至第9天开始产生HCG，在第9天至第12天，与Vero细胞共培养的胚胎的HCG分泌显著高于常规培养的胚胎（P < 0.03）。所获得囊胚的形态与其孵化和产生HCG的能力有关，但一种培养系统的囊胚形态并不比另一种培养系统的囊胚形态显著更好。
