Tachibana O, Lampe J, Kleihues P, Ohgaki H
Unit of Molecular Pathology, International Agency for Research on Cancer, Lyon, France.
Acta Neuropathol. 1996 Nov;92(5):431-4. doi: 10.1007/s004010050542.
Fas/APO-1 (CD95)-mediated apoptosis is one of the major mechanisms of programmed cell death. We have previously shown by reverse transcriptase-polymerase chain reaction that Fas is frequently expressed in malignant gliomas [Tachibana et al. (1995) Cancer Res 55: 5528-5530]. In this study, we assessed Fas expression in astrocytomas using a polyclonal anti-Fas antibody. Immunoreactivity to Fas was detected in 1 out of 9 (11%) low-grade astrocytomas (WHO grade II), 2 of 11 (18%) anaplastic astrocytomas (WHO grade III) and in 13 of 15 (87%) glioblastomas (WHO grade IV). In glioblastomas, Fas expression was almost exclusively observed in glioma cells surrounding foci of necrosis. In these perinecrotic areas, there was also an accumulation of glioma cells undergoing apoptosis, as detected by in situ nick-end labeling. This suggests that Fas-mediated apoptosis may play a role in the pathogenesis of necrosis which constitutes a histological hallmark of glioblastoma multiforme.
Fas/APO-1（CD95）介导的细胞凋亡是程序性细胞死亡的主要机制之一。我们之前通过逆转录聚合酶链反应表明，Fas在恶性胶质瘤中频繁表达[Tachibana等人（1995年），《癌症研究》55: 5528 - 5530]。在本研究中，我们使用多克隆抗Fas抗体评估了星形细胞瘤中Fas的表达。在9例低级别星形细胞瘤（WHO二级）中的1例（11%）、11例间变性星形细胞瘤（WHO三级）中的2例（18%）以及15例胶质母细胞瘤（WHO四级）中的13例（87%）检测到了对Fas的免疫反应性。在胶质母细胞瘤中，Fas表达几乎仅在坏死灶周围的胶质瘤细胞中观察到。在这些坏死周边区域，也有通过原位缺口末端标记检测到的正在经历凋亡的胶质瘤细胞的聚集。这表明Fas介导的细胞凋亡可能在坏死的发病机制中起作用，坏死是多形性胶质母细胞瘤的组织学特征。
