Gratas C, Tohma Y, Van Meir E G, Klein M, Tenan M, Ishii N, Tachibana O, Kleihues P, Ohgaki H
International Agency for Research on Cancer, France.
Brain Pathol. 1997 Jul;7(3):863-9. doi: 10.1111/j.1750-3639.1997.tb00889.x.
Fas/APO-1 (CD95) is a cell surface receptor that mediates apoptosis when it reacts with Fas ligand (FasL) or Fas antibody. We previously reported that Fas expression is predominantly induced in perinecrotic glioma cells, suggesting that Fas induction is associated with apoptosis and necrosis formation, a histological hallmark of glioblastomas. In this study, we assessed the expression of FasL in 10 glioblastoma cell lines and in 14 astrocytic brain tumors (three low-grade astrocytomas and 11 glioblastomas). Reverse transcriptase (RT)-PCR revealed that all glioblastoma cell lines and primary astrocytic brain tumors express FasL. Immunohistochemically, FasL was predominantly expressed on the plasma membrane of glioma cells. These results suggest that FasL expression is common in human astrocytic brain tumors and may cause apoptosis of glioma cells if Fas expression is induced.
Fas/APO-1（CD95）是一种细胞表面受体，当它与Fas配体（FasL）或Fas抗体反应时介导细胞凋亡。我们先前报道，Fas表达主要在坏死周围的胶质瘤细胞中被诱导，提示Fas诱导与凋亡和坏死形成相关，而凋亡和坏死形成是胶质母细胞瘤的组织学特征。在本研究中，我们评估了10种胶质母细胞瘤细胞系和14例星形细胞脑肿瘤（3例低级别星形细胞瘤和11例胶质母细胞瘤）中FasL的表达。逆转录酶（RT）-PCR显示，所有胶质母细胞瘤细胞系和原发性星形细胞脑肿瘤均表达FasL。免疫组织化学显示，FasL主要表达于胶质瘤细胞的质膜上。这些结果提示，FasL表达在人类星形细胞脑肿瘤中常见，并且如果Fas表达被诱导，可能导致胶质瘤细胞凋亡。
