Tsai C H, Tsai F J, Shih Y T, Wu S F, Liu S C, Tseng Y H
Department of Paediatrics, China Medical College Hospital, Taichung, Taiwan.
Acta Paediatr. 1996 Oct;85(10):1241-3. doi: 10.1111/j.1651-2227.1996.tb18237.x.
Polymerase chain reaction (PCR) amplification was used to detect cytomegalovirus (CMV) in 1000 urine specimens from Chinese newborns for defining the incidence of congenital CMV infection in the Chinese population. The major immediate-early and the late antigen genes of CMV were amplified and detected by gel electrophoresis. There were 18 congenitally infected infants found when tests were performed with one or both primer pairs. Comparing with tissue culture, PCR of both primer sets provided a sensitivity of 94%, a specificity of 100% and a predictive value of positive result of 100%.
采用聚合酶链反应（PCR）扩增技术检测1000份中国新生儿尿液标本中的巨细胞病毒（CMV），以确定中国人群中先天性CMV感染的发生率。通过凝胶电泳对CMV的主要即刻早期和晚期抗原基因进行扩增和检测。使用一对或两对引物进行检测时，共发现18例先天性感染婴儿。与组织培养相比，两组引物的PCR检测灵敏度为94%，特异性为100%，阳性结果预测值为100%。
