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Detection of congenital cytomegalovirus infection by using chorionic villi of the early pregnancy and polymerase chain reaction.

作者信息

Dong Z W, Yan C, Yi W, Cui Y Q

机构信息

Department of Genetics, National Research Institute for Family Planning, Beijing, China.

出版信息

Int J Gynaecol Obstet. 1994 Mar;44(3):229-31. doi: 10.1016/0020-7292(94)90171-6.

DOI:10.1016/0020-7292(94)90171-6
PMID:7909761
Abstract

OBJECTIVE

To detect congenital cytomegalovirus (CMV) infection of chorionic villi in early pregnancy.

METHODS

Extraction of DNA of chorionic villi and amplification of the gene of major immediate-early (MIE) antigen of CMV using a polymerase chain reaction (PCR).

RESULTS

Sixty-eight specimens of chorionic villi and 16 specimens were positive for CMV infection by PCR. The incidence of congenital CMV infection in the first trimester of pregnancy was 23.5%.

CONCLUSIONS

The risk of transmission of CMV from mother to fetus in early pregnancy is very high and potential CMV carriers may transmit CMV to their fetus in early pregnancy.

摘要

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