Zhang Q Q, Ren L, Wang C, Liu L R, Wen X J, Liu Y H, Zhang X D
Department of Biomaterials, Chinese Academy of Medical Sciences, Tianjin, P.R. China.
Artif Cells Blood Substit Immobil Biotechnol. 1996 Nov;24(6):693-702. doi: 10.3109/10731199609118892.
Porous hydroxyapatite (HAP) with certain porosity and pore size was prepared, and incorporated with bovine collagen protein. The composition and structure of the HAP was confirmed by X-Ray Diffraction (XRD) and ICP. Scanning Electron Microscopy (SEM), mechanical tests and in vitro degradation were performed. Collagen protein with low antigenicity was obtained from bovine tendon by enzyme digestion, and was then forced to fill in the HAP matrix to form composites. Scanning Electron Microscopy (SEM), Mechanical tests and in vitro degradation were performed. The test results show that first, HAP thus made has specific pore size and directions; second, mechanical properties of the composites have been markedly improved; third, the in vitro degradation rate of the composite is almost the same as and mainly controlled by the degradation rate of collagen.
制备了具有一定孔隙率和孔径的多孔羟基磷灰石（HAP），并将其与牛胶原蛋白相结合。通过X射线衍射（XRD）和电感耦合等离子体质谱（ICP）确定了HAP的组成和结构。进行了扫描电子显微镜（SEM）、力学测试和体外降解实验。通过酶消化从牛肌腱中获得低抗原性的胶原蛋白，然后将其填充到HAP基质中以形成复合材料。进行了扫描电子显微镜（SEM）、力学测试和体外降解实验。测试结果表明，首先，如此制备的HAP具有特定的孔径和方向；其次，复合材料的力学性能得到了显著改善；第三，复合材料的体外降解速率几乎与胶原蛋白的降解速率相同，且主要受胶原蛋白降解速率的控制。
