National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, People's Republic of China.
Biofabrication. 2014 Sep;6(3):035013. doi: 10.1088/1758-5082/6/3/035013. Epub 2014 May 30.
A three-level hierarchical calcium phosphate/collagen/hydroxyapatite (CaP/Col/HAp) scaffold for bone tissue engineering was developed using biomimetic synthesis. Porous CaP ceramics were first prepared as substrate materials to mimic the porous bone structure. A second-level Col network was then composited into porous CaP ceramics by vacuum infusion. Finally, a third-level HAp layer was achieved by biomimetic mineralization. The three-level hierarchical biomimetic scaffold was characterized using scanning electron microscopy, energy-dispersive x-ray spectra, x-ray diffraction and Fourier transform infrared spectroscopy, and the mechanical properties of the scaffold were evaluated using dynamic mechanical analysis. The results show that this scaffold exhibits a similar structure and composition to natural bone tissues. Furthermore, this three-level hierarchical biomimetic scaffold showed enhanced mechanical strength compared with pure porous CaP scaffolds. The biocompatibility and osteoinductivity of the biomimetic scaffolds were evaluated using in vitro and in vivo tests. Cell culture results indicated the good biocompatibility of this biomimetic scaffold. Faster and increased bone formation was observed in these scaffolds following a six-month implantation in the dorsal muscles of rabbits, indicating that this biomimetic scaffold exhibits better osteoinductivity than common CaP scaffolds.
采用仿生合成的方法制备了具有 3 级结构的磷酸钙/胶原/羟基磷灰石（CaP/Col/HAp）仿生骨组织工程支架。首先制备多孔 CaP 陶瓷作为基底材料，以模拟多孔骨结构。然后通过真空浸渍将第二级 Col 网络复合到多孔 CaP 陶瓷中。最后，通过仿生矿化获得第三级 HAp 层。采用扫描电子显微镜、能谱、X 射线衍射和傅里叶变换红外光谱对 3 级仿生支架进行了表征，并采用动态力学分析对支架的力学性能进行了评价。结果表明，该支架具有与天然骨组织相似的结构和组成。此外，与纯多孔 CaP 支架相比，这种 3 级仿生支架具有更高的机械强度。通过体外和体内试验评价了仿生支架的生物相容性和成骨诱导性。细胞培养结果表明该仿生支架具有良好的生物相容性。在兔背部肌肉中植入 6 个月后，观察到这些支架中更快和更多的骨形成，表明该仿生支架比普通 CaP 支架具有更好的成骨诱导性。
