Holt P R, Moss S F, Whelan R, Guss J, Gilman J, Lipkin M
Gastrointestinal Division, St. Luke's-Roosevelt Hospital Center, New York, New York 10025, USA.
Cancer Epidemiol Biomarkers Prev. 1996 Nov;5(11):937-40.
Fecal diacylglycerol (DAG) concentrations have been suggested as biomarkers for colonic neoplasia because of their potential to be absorbed in the colon and to stimulate epithelial cell proliferation. The interrelationships among nutrient intake, fecal and mucosal DAG, and colonic proliferative markers have not previously been studied. We designed a pilot study to evaluate the feasibility of evaluating these interrelationships in 12 volunteers who had a history of colonic adenomatous polyposis. Total mucosal DAG concentrations were not related to fecal DAG concentrations, but mucosal DAG correlated inversely with the whole crypt labeling index. Dietary intake did not alter fecal DAG concentrations. However, the percentage of calories from dietary fat correlated positively with the whole crypt labeling index. Fiber and calcium intake showed a positive correlation with the labeling index in the upper 40% of the crypt. The present pilot study failed to demonstrate a correlation between dietary components and fecal and total mucosal DAG. Additional studies relating fecal DAG with mucosal proliferation will require the evaluation of DAG concentrations in subcellular compartments of mucosal cells and/or measurement of fecal DAG fatty acid composition.
粪便二酰甘油（DAG）浓度被认为是结肠肿瘤的生物标志物，因为它们有可能在结肠中被吸收并刺激上皮细胞增殖。营养摄入、粪便和黏膜DAG以及结肠增殖标志物之间的相互关系此前尚未得到研究。我们设计了一项初步研究，以评估在12名有结肠腺瘤性息肉病史的志愿者中评估这些相互关系的可行性。黏膜DAG总浓度与粪便DAG浓度无关，但黏膜DAG与整个隐窝标记指数呈负相关。饮食摄入并未改变粪便DAG浓度。然而，饮食脂肪中的卡路里百分比与整个隐窝标记指数呈正相关。纤维和钙的摄入量与隐窝上部40%的标记指数呈正相关。目前的初步研究未能证明饮食成分与粪便及总黏膜DAG之间存在相关性。将粪便DAG与黏膜增殖相关联的进一步研究将需要评估黏膜细胞亚细胞区室中的DAG浓度和/或测量粪便DAG脂肪酸组成。
