Erichsen S, Zuo J, Curtis L, Rarey K, Hultcrantz M
Department of Otorhinolaryngology, Karolinska Hospital, Stockholm, Sweden.
Hear Res. 1996 Oct;100(1-2):143-9. doi: 10.1016/0378-5955(96)00105-0.
Immunohistochemistry was used to investigate the presence of Na,K-ATPase alpha- and beta-subunits isoforms (alpha 1, alpha 2, alpha 3, beta 1 and beta 2) in the cochlea of the mouse at different ages between embryological day (E) 19 and postnatal day (P) + 30. alpha 1 was mainly found in the stria vascularis and in the spiral ligament; it increased steadily from p+4. These data correlates well with the morphological and electrophysiological maturation of the cochlea. alpha 3 predominated in the spiral ganglia and the cochlear nerve. This finding is well in accordance with reports that alpha 3 seems to be associated with the nervous system. The beta-subunit was found mainly in those tissues where staining of the alpha-subunit also was seen. Both subunits were localized in tissue regions where fluid regulation is expected to play an important role. For some isoforms, the expression pattern of Na,K-ATPase during development in the mouse is different from that in the rat. The expression of Na,K-ATPase and that of glucocorticoid receptors during development in the inner ear of the mouse show a similar pattern, which may indicate that glucocorticoid receptors could be involved in regulating the expression of Na,K-ATPase.
采用免疫组织化学方法研究了胚胎期第19天（E19）至出生后第30天（P + 30）不同年龄段小鼠耳蜗中Na，K - ATP酶α亚基和β亚基同工型（α1、α2、α3、β1和β2）的存在情况。α1主要存在于血管纹和螺旋韧带中；从出生后第4天开始稳步增加。这些数据与耳蜗的形态学和电生理成熟情况密切相关。α3在螺旋神经节和蜗神经中占主导地位。这一发现与α3似乎与神经系统相关的报道非常一致。β亚基主要存在于那些也可见α亚基染色的组织中。两个亚基都定位于预期液体调节起重要作用的组织区域。对于某些同工型，小鼠发育过程中Na，K - ATP酶的表达模式与大鼠不同。小鼠内耳发育过程中Na，K - ATP酶和糖皮质激素受体的表达呈现相似模式，这可能表明糖皮质激素受体可能参与调节Na，K - ATP酶的表达。
