Wildemauwe C, Godard C, Vanhoof R, Bossuyt E V, Hannecart-Pokorni E
Pasteur Institute-Brussels, Belgium.
J Hosp Infect. 1996 Nov;34(3):197-203. doi: 10.1016/s0195-6701(96)90066-3.
A total of 102 epidemic methicillin-resistant Staphylococcus aureus (MRSA) isolates collected in 13 Belgian hospitals during two periods (1981-1985 and 1991-1992) were tested for phage-type, for the presence of aminoglycoside-modifying enzymes (AME), and examined by arbitrarily primed polymerase chain reaction (AP-PCR). All isolates, but five, belonged to a few distinct phage-types of group III. Most isolates expressed a combination of AAC(6')-APH(2") with APH(3')III, and ANT(4',4") or both. Both phage-typing and AME suggested a change in the MRSA population between the two periods but the AP-PCR method revealed only slight differences.
在两个时间段（1981 - 1985年和1991 - 1992年）从比利时13家医院收集的总共102株耐甲氧西林金黄色葡萄球菌（MRSA）分离株，进行了噬菌体分型、氨基糖苷类修饰酶（AME）检测，并通过随机引物聚合酶链反应（AP-PCR）进行分析。除了5株分离株外，所有分离株都属于III组的几种不同噬菌体类型。大多数分离株表达了AAC(6')-APH(2")与APH(3')III以及ANT(4',4")的组合，或同时表达两者。噬菌体分型和AME检测均表明两个时间段之间MRSA菌群发生了变化，但AP-PCR方法仅显示出细微差异。
