Vanhoof R, Godard C, Content J, Nyssen H J, Hannecart-Pokorni E
Pasteur Institute of Brabant, Brussels, Belgium.
J Med Microbiol. 1994 Oct;41(4):282-90. doi: 10.1099/00222615-41-4-282.
The polymerase chain reaction (PCR) was used to identify the aacA-aphD, aphA3 and aadC genes, encoding the aminoglycoside-modifying enzymes AAC(6')-APH(2"), APH(3')III and ANT(4'4"), respectively, and the methicillin resistance determinant mecA, in epidemic aminoglycoside and methicillin-resistant isolates of Staphylococcus aureus. In total, 37 isolates collected in the period 1980-1985 and 81 isolates from the period 1991-1992 were obtained from 10 different Belgian hospitals. Epidemic isolates from the earlier period were characterised by phage type C (6/47/54/75) of phage group III, whereas two other epidemic phage types of group III-types A (77) and B (47/54/75/77/84/85)--were commonest in isolates from the second period. The bifunctional AAC(6')-APH(2") was the enzyme encountered most frequently. The prevalence of APH(3')III decreased significantly in the 1991-1992 period, while ANT(4',4") was found solely in isolates from this period. Resistance mechanisms were more complex in isolates from the 1991-1992 period and the mecA gene was detected in all isolates. The PCR results corresponded well with those obtained in the radiochemical phosphocellulose paper binding assay. Isolates from the 1991-1992 period were shown to express significantly higher levels of acetyltransferase activity than isolates from the 1980s.
采用聚合酶链反应（PCR）鉴定编码氨基糖苷修饰酶AAC(6')-APH(2")、APH(3')III和ANT(4'4")的aacA-aphD、aphA3和aadC基因，以及耐甲氧西林金黄色葡萄球菌流行株中的耐甲氧西林决定簇mecA。总共从比利时10家不同医院获得了1980 - 1985年期间收集的37株菌株和1991 - 1992年期间的81株菌株。早期的流行株以III组噬菌体C型（6/47/54/75）为特征，而III组的另外两种流行噬菌体类型——A型（77）和B型（47/54/75/77/84/85）——在第二个时期的分离株中最为常见。双功能酶AAC(6')-APH(2")是最常遇到的酶。1991 - 1992年期间APH(3')III的流行率显著下降，而ANT(4',4")仅在该时期的分离株中发现。1991 - 1992年期间分离株的耐药机制更为复杂，并且在所有分离株中均检测到mecA基因。PCR结果与放射化学磷酸纤维素纸结合试验的结果非常吻合。结果表明，1991 - 1992年期间的分离株比20世纪80年代的分离株表达出显著更高水平的乙酰转移酶活性。
