Yiin S J, Lin T H, Shih T S
Ta Jen Junior College of Pharmacy, Pintung, Taiwan, Republic of China.
Scand J Work Environ Health. 1996 Oct;22(5):381-6. doi: 10.5271/sjweh.158.
The following hypothesis was tested: plasma manganese concentration is associated positively with the product of lipid peroxidation, and lipid peroxidation is associated negatively with the activities of antioxidants in workers exposed to manganese.
The plasma manganese concentration of 22 manganese-exposed workers and 45 referents was determined by graphite furnace atomic absorption spectrophotometry. Malondialdehyde, the product of lipid peroxidation, was determined by high-performance liquid chromatography, and the activities of protective enzymes were measured by ultraviolet-visible spectrophotometry.
The activities of superoxide dismutase, glutathione peroxidase, and catalase spread widely among the referents. The activity of superoxide dismutase and the concentrations of malondialdehyde and manganese were significantly higher in the manganese workers than in the referents. The concentration of malondialdehyde in the exposed workers was correlated strongly with the manganese level of plasma.
Malondialdehyde can be used as an index of lipid peroxidation induced by manganese exposure.
对以下假设进行了验证：血浆锰浓度与脂质过氧化产物呈正相关，而脂质过氧化与接触锰的工人体内抗氧化剂的活性呈负相关。
采用石墨炉原子吸收分光光度法测定了22名接触锰的工人和45名对照者的血浆锰浓度。通过高效液相色谱法测定脂质过氧化产物丙二醛，并采用紫外可见分光光度法测定保护酶的活性。
超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶的活性在对照者中分布广泛。锰接触工人中超氧化物歧化酶的活性以及丙二醛和锰的浓度显著高于对照者。接触工人中丙二醛的浓度与血浆锰水平密切相关。
丙二醛可作为锰暴露诱导脂质过氧化的指标。
