采用毛细管气相色谱 - 质谱联用技术对人体组织中溴西泮进行灵敏测定。

Sensitive determination of bromazepam in human tissues using capillary gas chromatography-mass spectrometry.

作者信息

Zhang X X, Kudo K, Imamura T, Jitsufuchi N, Nagata T

机构信息

Department of Forensic Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.

出版信息

J Chromatogr B Biomed Appl. 1996 Feb 23;677(1):111-6. doi: 10.1016/0378-4347(95)00427-0.

DOI:10.1016/0378-4347(95)00427-0

PMID:8925082

Abstract

A reliable and sensitive gas chromatographic-mass spectrometric method was devised to determine the levels of bromazepam in human tissues. Bromazepam was extracted from body tissues using a three-step solvent extraction procedure. N-Desmethyldiazepam served as the internal standard. Selected ion monitoring with m/z 317 for bromazepam and m/z 270 for internal standard was used for quantitation. Calibration curves in all body tissues were linear over the concentration range from 50-500 ng/g. The lower detection limit in body tissues was 2-5 ng/g and the absolute recovery in body tissues was 27.8-68.0%. This method was used to determine the levels of bromazepam in tissues of an autopsied individual who had been prescribed psychotropic drugs and who was found dead in a car.

摘要

设计了一种可靠且灵敏的气相色谱 - 质谱法来测定人体组织中溴西泮的含量。采用三步溶剂萃取程序从人体组织中提取溴西泮。N - 去甲基地西泮作为内标。使用对溴西泮选择m/z 317、对内标选择m/z 270的选择离子监测进行定量。所有人体组织中的校准曲线在50 - 500 ng/g的浓度范围内呈线性。人体组织中的最低检测限为2 - 5 ng/g，人体组织中的绝对回收率为27.8 - 68.0%。该方法用于测定一名服用过精神药物且被发现死于车内的尸检个体组织中溴西泮的含量。