Thompson M J, Ballinger L N, Cross S E, Roberts M S
Department of Medicine, University of Queensland, Princess Alexandra Hospital, Brisbane, Australia.
J Chromatogr B Biomed Appl. 1996 Feb 23;677(1):117-22. doi: 10.1016/0378-4347(95)00368-1.
This paper describes a simple and more sensitive reversed-phase HPLC method for the quantification of phenol, 4-nitrophenol and beta-naphthol and some of their glucuronide and sulphate conjugates in aqueous solution and liver perfusate buffer. Methanol-water mobile phases with ion-pairing agents for each phenolic group are detailed. The assay showed good recovery, accuracy and precision and is suitable for the quantification of these phenolic compounds in liver perfusion experiments.
本文描述了一种简单且更灵敏的反相高效液相色谱法，用于定量分析水溶液和肝脏灌注缓冲液中的苯酚、4-硝基苯酚和β-萘酚及其一些葡萄糖醛酸和硫酸盐共轭物。详细介绍了针对每个酚类基团使用离子对试剂的甲醇-水流动相。该测定方法具有良好的回收率、准确性和精密度，适用于肝脏灌注实验中这些酚类化合物的定量分析。
