Zavizion B, van Duffelen M, Schaeffer W, Politis I
Department of Animal and Food Sciences, University of Vermont, Burlington 05405, USA.
In Vitro Cell Dev Biol Anim. 1996 Mar;32(3):138-48. doi: 10.1007/BF02723679.
Clonal cell lines (BME-UV) were established from primary epithelial cells by stable transfection with a plasmid, carrying the sequence of the simian virus 40 early region mutant tsA58, encoding the thermolabile large T antigen. The BME-UV cells have undergone more than 300 population doublings and produce intranuclear large T antigen. At low confluency, growing islands of cells are apparent exhibiting the characteristic cobblestone morphology of epithelial cells. The BME-UV cells expressed functional markers such as microvilli and desmosomes and biochemical markers of mammary epithelial cells such as a repertoire of cytokeratins. The BME-UV cells are capable of synthesizing low levels of alpha-lactalbumin and alpha s1-casein (50 ng/ml of medium/24 h). One of the cell lines, BME-UV1 showed enhanced proliferation in the presence of epidermal growth factor (EGF) and insulinlike growth factor I (IGF-I). The BME-UV1 cell line is the only known bovine mammary epithelial cell line responsive to EGF. The BME-UV cells grown on collagen at low confluency are capable of developing very long projections that most likely allow for communication between cells at a distance from each other. The BME-UV cells may become a valid model system to examine bovine mammary epithelial proliferation and differentiation and cell-to-cell communication.
通过用携带猿猴病毒40早期区域突变体tsA58序列（编码热不稳定大T抗原）的质粒对原代上皮细胞进行稳定转染，建立了克隆细胞系（BME-UV）。BME-UV细胞已经历了300多次群体倍增，并产生核内大T抗原。在低汇合度时，明显可见生长的细胞岛，呈现出上皮细胞典型的鹅卵石形态。BME-UV细胞表达了微绒毛和桥粒等功能标记以及乳腺上皮细胞的生化标记，如细胞角蛋白谱。BME-UV细胞能够合成低水平的α-乳白蛋白和αs1-酪蛋白（每毫升培养基50纳克/24小时）。其中一个细胞系BME-UV1在表皮生长因子（EGF）和胰岛素样生长因子I（IGF-I）存在的情况下增殖增强。BME-UV1细胞系是唯一已知的对EGF有反应的牛乳腺上皮细胞系。在低汇合度下生长在胶原蛋白上的BME-UV细胞能够形成非常长的突起，这很可能有助于远距离细胞之间的通讯。BME-UV细胞可能成为研究牛乳腺上皮细胞增殖、分化和细胞间通讯的有效模型系统。
