Proust J, Houlné G, Schantz M L, Schantz R
Institut de Biologie Moleculaire des Plantes, CNRS, Universite Louis Pasteur, France.
FEBS Lett. 1996 Apr 1;383(3):208-12. doi: 10.1016/0014-5793(96)00252-9.
Several lines of evidence indicate that annexins, as calcium-dependent phospholipid-binding proteins, are involved in a variety of plant cellular processes. We were interested in determining if annexins are implicated in the highly regulated fruit development of bell pepper. By differential screening of several cDNA libraries, we isolated a full-length cDNA of 1180 bp encoding an annexin. Northern blot analyses show a differential expression pattern of the transcripts during the early stages of development and during ripening. Immunoblots using antiserum raised against p33/p35 from maize reveal that cross-reactive polypeptides of about 30 kDa are present at each stage of fruit development in bell pepper. We partially purified the annexins from seedlings and green fruits. At least one annexin of 32 kDa is present in these plant tissues.
多项证据表明，膜联蛋白作为钙依赖性磷脂结合蛋白，参与了多种植物细胞过程。我们感兴趣的是确定膜联蛋白是否与甜椒高度受控的果实发育有关。通过对几个cDNA文库的差异筛选，我们分离出了一个编码膜联蛋白的1180 bp全长cDNA。Northern印迹分析表明，转录本在发育早期和成熟过程中呈现出差异表达模式。使用针对玉米p33/p35产生的抗血清进行的免疫印迹显示，在甜椒果实发育的每个阶段都存在约30 kDa的交叉反应性多肽。我们从幼苗和绿色果实中部分纯化了膜联蛋白。这些植物组织中至少存在一种32 kDa的膜联蛋白。
