Mizuno S, Ikeda K, Hirabayashi Y
Department of Ophthalmology, Nagoya City University Medical School, Japan.
Jpn J Ophthalmol. 1995;39(4):340-6.
Histochemical studies were carried out in the mouse on the changes and distribution patterns of glycosaminoglycan molecular species during the separation of the lens vesicle. To obtain embryos, pregnant mice of the Jcl:ICR strain were sacrificed on day 10.5 or 11 of pregnancy. Serial frontal sections were strained with hematoxylin-eosin and sensitized high iron diamine. To identify glycosaminoglycan molecular species in tissues, an enzyme digestion (double digestion with chondroitinase B and testicular hyaluronidase) and a chemical modification (nitrous acid treatment) were performed in combination with the sensitized high iron diamine method. Before separation of the lens vesicle, chondroitin sulfate A/C and B were found in the basement membrane of the future corneal epithelium and intercellular matrices between the future corneal epithelium and lens vesicle, and chondroitin sulfate A/C was identified in the lens capsule. After separation of the lens vesicle, heparan sulfate emerged in the basement membrane of the future corneal epithelium and intercellular matrices between the future corneal epithelium and lens vesicle. These results indicate that the changes and distribution pattern of glycosaminoglycan molecular species play an important role during the separation of the lens vesicle.
在小鼠中进行了组织化学研究，以观察晶状体泡分离过程中糖胺聚糖分子种类的变化和分布模式。为了获取胚胎，将Jcl:ICR品系的怀孕小鼠在妊娠第10.5天或第11天处死。连续额状切片用苏木精-伊红染色并进行高铁二胺敏化。为了鉴定组织中的糖胺聚糖分子种类，结合高铁二胺敏化法进行了酶消化（用硫酸软骨素酶B和睾丸透明质酸酶双重消化）和化学修饰（亚硝酸处理）。在晶状体泡分离之前，硫酸软骨素A/C和B存在于未来角膜上皮的基底膜以及未来角膜上皮与晶状体泡之间的细胞间基质中，并且在晶状体囊膜中鉴定出硫酸软骨素A/C。在晶状体泡分离之后，硫酸乙酰肝素出现在未来角膜上皮的基底膜以及未来角膜上皮与晶状体泡之间的细胞间基质中。这些结果表明，糖胺聚糖分子种类的变化和分布模式在晶状体泡分离过程中起重要作用。
