Ikeda K, Hirabayashi Y
Department of Ophthalmology, Nagoya City University Medical School, Japan.
Jpn J Ophthalmol. 1992;36(4):388-400.
Histological and histochemical studies have been made on the microscopic structures of the ciliary body from the developing eyes of both pre- (on day 13, 16 and 18 of gestation) and postnatal (on day 1, 5, 10, 14 and 84) mice of the Jcl:ICR strain and on the acidic glycoconjugates contained in the ciliary body tissues of these animals. As the staining procedures of choice, a hematoxylin-eosin procedure and a sensitized high iron diamine method were employed. A nitrous acid procedure was used in combination for some tissue sections, prior to the sensitized diamine method, to aid in the identification of sulfated glycoconjugates. Both the partes optica and caeca retinae could be identified histochemically as early as day 13 of gestation, whereas these parts could not be histologically distinguished from each other at day 16 of gestation. The ciliary folds were detected first in the external layer at day 16 of gestation and subsequently in the internal layer 2 days later. The heparan sulphate proteoglycan observed not only in the thick basement membrane but also in the intra- and intercellular spaces of the internal layer was localized in a distribution pattern which prevented the occurrence of configurational discrepancies between the epithelial cells lining the layer because of the formation of ciliary folds.
对Jcl:ICR品系的产前（妊娠第13、16和18天）和产后（出生第1、5、10、14和84天）小鼠发育眼睛的睫状体微观结构及其睫状体组织中所含酸性糖缀合物进行了组织学和组织化学研究。作为首选的染色方法，采用了苏木精-伊红染色法和敏化高铁二胺法。在敏化二胺法之前，对一些组织切片联合使用亚硝酸法，以帮助鉴定硫酸化糖缀合物。早在妊娠第13天，光学部和视网膜盲部就可以通过组织化学方法鉴定出来，而在妊娠第16天，这些部分在组织学上无法相互区分。睫状褶在妊娠第16天首先在外层被检测到，随后在2天后在内层被检测到。硫酸乙酰肝素蛋白聚糖不仅存在于厚基底膜中，也存在于内层的细胞内和细胞间空间，其定位分布模式可防止由于睫状褶的形成而导致该层内衬上皮细胞之间出现构型差异。
