Mizuno S, Ikeda K, Majima A, Hirabayashi Y, Yamada K
Department of Ophthalmology, Nagoya City University Medical School, Aichi-ken, Japan.
Nippon Ganka Gakkai Zasshi. 1997 Jan;101(1):46-51.
We studied histologically the changes and distribution patterns of glycosaminoglycan molecular species during the separation of the lens vesicle in the mouse. Embryos were obtained by sacrificing pregnant mice of the Jcl: ICR strain on day 10.5 and 11 of pregnancy. Serial frontal sections were stained with hematoxylin-eosin and a sensitized high iron diamine method. To identify glycosaminoglycan molecular species in tissues, enzyme digestion (double digestions with chondroitinase B and testicular hyaluronidase) and chemical modification (nitrous acid treatment) were performed in combination with the sensitized high iron diamine method. Before separation of the lens vesicle, the glycosaminoglycan molecular species, identified in the basement membrane of the presumed corneal epithelium and intercellular matrices between the presumed corneal epithelium and lens vesicle, were chondroitin sulfate A/C and B, and those in the lens capsule were chondroitin sulfate A/C. After separation of the lens vesicle, heparan sulfate emerged in the basement membrane of the presumed corneal epithelium and intercellular matrices between the presumed corneal epithelium and lens vesicle. These results are thus taken to indicate that the changes and distribution patterns of glycosaminoglycan molecular species play an important role during separation of the lens vesicle.
我们对小鼠晶状体泡分离过程中糖胺聚糖分子种类的变化和分布模式进行了组织学研究。在妊娠第10.5天和第11天处死Jcl: ICR品系的怀孕小鼠以获取胚胎。连续额状切片用苏木精-伊红染色和敏化高铁二胺法染色。为了鉴定组织中的糖胺聚糖分子种类，结合敏化高铁二胺法进行酶消化（用软骨素酶B和睾丸透明质酸酶双重消化）和化学修饰（亚硝酸处理）。在晶状体泡分离前，在假定的角膜上皮基底膜以及假定的角膜上皮与晶状体泡之间的细胞间基质中鉴定出的糖胺聚糖分子种类为硫酸软骨素A/C和B，而在晶状体囊膜中的为硫酸软骨素A/C。晶状体泡分离后，硫酸乙酰肝素出现在假定的角膜上皮基底膜以及假定的角膜上皮与晶状体泡之间的细胞间基质中。因此，这些结果表明糖胺聚糖分子种类的变化和分布模式在晶状体泡分离过程中起重要作用。
