Brière F, Chevet D, Bridon J M, Souillet G, Rifle G, Banchereau J
Schering-Plough, Laboratory for Immunological Research, Dardilly.
Nephrologie. 1996;17(5):289-95.
We have previously shown that human B lymphocytes cultured in the CD40 system, composed of an anti-CD40 mAb presented by a CD32-transfected fibroblastic cell line, proliferate but do not secrete immunoglobulins (Igs). However, the addition of particles of Staphylococcus aureus Cowan (SAC) induces B cell to secrete considerable amounts of Igs even in the absence of exogenous cytokines (CD40/SAC system). Additionally, B lymphocytes cultured in the CD40 system in the presence of human IL-10, produce high level of IgM, IgG and IgA, which are further increased by addition of SAC. Here, we have studied the capacity of peripheral blood lymphocytes from patients with IgA deficiency (IgA-D) to secrete Igs, particularly IgA after CD40 triggering. Peripheral blood mononuclear cells (PBMNC) from IgA-D patients cultured in the CD40/SAC system produced IgM and IgG, but no IgA. The addition of IL-10 to the cultures, enhanced the production of IgM and IgG and most strikingly induced the production of high amounts of IgA. The addition of IL-10 to PBMNC from IgA-D patients activated through CD40 alone resulted in the production of IgA. Thus, IL-10 can remove the block in B cell differentiation and allows B cells from IgA-D patients to differentiate into IgA secreting cells.
我们之前已经表明，在由CD32转染的成纤维细胞系呈递的抗CD40单克隆抗体组成的CD40系统中培养的人B淋巴细胞会增殖，但不分泌免疫球蛋白（Ig）。然而，添加金黄色葡萄球菌考恩株（SAC）颗粒即使在没有外源性细胞因子的情况下（CD40/SAC系统）也能诱导B细胞分泌大量Ig。此外，在人IL-10存在的情况下，在CD40系统中培养的B淋巴细胞会产生高水平的IgM、IgG和IgA，添加SAC后这些水平会进一步升高。在此，我们研究了IgA缺乏症（IgA-D）患者外周血淋巴细胞分泌Ig，特别是CD40触发后分泌IgA的能力。在CD40/SAC系统中培养的IgA-D患者的外周血单个核细胞（PBMNC）产生IgM和IgG，但不产生IgA。向培养物中添加IL-10可增强IgM和IgG的产生，最显著的是诱导产生大量IgA。向仅通过CD40激活的IgA-D患者的PBMNC中添加IL-10会导致IgA的产生。因此，IL-10可以消除B细胞分化中的障碍，并使IgA-D患者的B细胞分化为分泌IgA的细胞。
