Sunardhi-Widyaputra S, Van Damme B
Department of Pathology II, Sint Raphaël University Hospital, Leuven, Belgium.
Pathol Res Pract. 1995 Dec;191(12):1186-91. doi: 10.1016/S0344-0338(11)81124-6.
Forty-seven samples of paraffin-embedded formalin-fixed (and 25 related frozen) sections of 27 primary pleomorphic adenomas, 15 recurrent pleomorphic adenomas and 5 carcinomas in pleomorphic adenomas were studied to analyse their immunohistologic patterns with respect to the ratio of the expression of 'normally' and 'aberrantly' differentiated cell types. In primary pleomorphic adenoma PTHrP-positive cells are seen in the inner layer of tubulo-ductal structures, in part of the cells in the mucoid, chondroid, or myxochondroid matrix, and in the squamous metaplastic areas. Bcl-2-positive cells are found in the outer layer of tubulo-ductal structures, in part of the cells in the mucoid, chondroid, or myxochondroid matrix, and around the squamous metaplastic areas. In one case of primary pleomorphic adenoma, which recurred later, the positivity for Bcl-2 is more intense and seen in the periphery of this tumour with a predominantly myxoid pattern. In recurrent pleomorphic adenomas, which also mostly showed a predominantly myxoid pattern, the positivity for Bcl-2 showed a pattern similar to the primary-to-recur tumour. PTHrP-positive cells are found less frequently than Bcl-2-positive cells. In carcinoma in pleomorphic adenoma, the benign part shows the features of primary pleomorphic adenoma with its Bcl-2 and PTHrP-positivity patterns. The malignant part strongly shows Bcl-2-positive cells in the periphery of the tumour. We conclude that the maintained presence of Bcl-2 and PTHrP-positive cells in the tumours we studied shows the variable capacity of tumour cells to differentiate.
对27例原发性多形性腺瘤、15例复发性多形性腺瘤和5例多形性腺瘤癌变的47个石蜡包埋福尔马林固定（以及25个相关冰冻）切片样本进行研究，以分析其免疫组织学模式，观察“正常”和“异常”分化细胞类型表达比例。在原发性多形性腺瘤中，甲状旁腺激素相关蛋白（PTHrP）阳性细胞见于小管-导管结构的内层、黏液样、软骨样或黏液软骨样基质中的部分细胞以及鳞状化生区域。Bcl-2阳性细胞见于小管-导管结构的外层、黏液样、软骨样或黏液软骨样基质中的部分细胞以及鳞状化生区域周围。在1例后来复发的原发性多形性腺瘤中，Bcl-2阳性更强，在以黏液样为主的肿瘤周边可见。在复发性多形性腺瘤中，大多也以黏液样为主，Bcl-2阳性模式与原发性至复发性肿瘤相似。PTHrP阳性细胞比Bcl-2阳性细胞少见。在多形性腺瘤癌变中，良性部分呈现原发性多形性腺瘤的特征，具有其Bcl-2和PTHrP阳性模式。恶性部分在肿瘤周边强烈显示Bcl-2阳性细胞。我们得出结论，在我们研究的肿瘤中Bcl-2和PTHrP阳性细胞的持续存在表明肿瘤细胞具有不同的分化能力。
