Tylewska-Wierzbanowska S, Kruszewska D, Chmielewski T
Zakład Bakteriologii Państwowego Zakładu Higieny w Warszawie.
Przegl Epidemiol. 1996;50(3):239-44.
Material samples from 40 ticks, including 30 Ixodes ricinus and 10 Dermacentor reticulatus ticks were tested for presence of Borrelia burgdorferi infection with indirect immunofluorescence assay (IF) and polymerase chain reaction (PCR). Sensitivity of PCR in terms of minimum detectable number of in vitro cultivated spirochetes was a reproducible amplification when 50 spirochetes were added to the PCR mixture. The assay sensitivity was lower for B. burgdorferi in ticks and it was estimated on minimum 100 bacterial cells. B. burgdorferi DNA (16S rDNA) has been found in 10 I. ricinus ticks. Immunofluorescence has been observed in Immunofluorescence has been observed in 32 samples derived from 22 I. ricinus and 10 D. reticulatus ticks both incubated with immune serum for B. Burgdorferi as well as with normal serum. Our results indicate that due to very low specificity IF is not a usefull method for testing of tick material.
对40只蜱虫的材料样本进行了检测，其中包括30只蓖麻硬蜱和10只血红扇头蜱，采用间接免疫荧光法（IF）和聚合酶链反应（PCR）检测伯氏疏螺旋体感染情况。就体外培养螺旋体的最低可检测数量而言，PCR的灵敏度是指当向PCR混合物中加入50个螺旋体时可实现可重复扩增。该检测方法对蜱虫中伯氏疏螺旋体的灵敏度较低，估计至少需要100个细菌细胞。在10只蓖麻硬蜱中发现了伯氏疏螺旋体DNA（16S rDNA）。在来自22只蓖麻硬蜱和10只血红扇头蜱的32个样本中均观察到了免疫荧光，这些样本既与抗伯氏疏螺旋体免疫血清孵育，也与正常血清孵育。我们的结果表明，由于特异性非常低，免疫荧光法不是检测蜱虫材料的有用方法。
