Stańczak J, Racewicz M, Kubica-Biernat B, Kruminis-Lozowska W, Dabrowski J, Adamczyk A, Markowska M
Department of Tropical Parasitology, Institute of Maritime and Tropical Medicine, Powstania Styczniowego 9B, 81-519 Gdynia, Poland.
Ann Agric Environ Med. 1999;6(2):127-32.
In 1996-1998, a total of 2285 Ixodes ricinus ticks (1063 nymphs, 637 males, 585 females) were collected from vegetation from 25 different localities in the 8 Polish provinces throughout the country. Ticks inhabited all 25 collection sites. The average number of ticks per collection site was 91.4 +/- 13.7. All 2285 ticks were examined for Borrelia burgdorferi sensu lato (s.l.) presence, of which 1333 specimens from 3 provinces were tested by routine indirect immunofluorescence assay (IFA) using polyclonal antibody PAB 1B29. The remaining 952 specimens from 5 provinces were examined by polymerase chain reaction (PCR), using FL6 and FL7 primers. The overall infection rate in ticks estimated by these 2 methods was 10. 2%. Nymphs showed lower positivity rate (6.2%) as compared to adult ticks (14.9% in females and 12.4% in males). The highest percentage of infected I. ricinus ticks (37.5%) was noted in the Katowice province while the lowest (4.1%) in the Bia ystok province. In particular collection sites, infection rates varied from 0-37.5%. The obtained results confirmed that B. burgdorferi s.l. is present throughout the distributional areas of I. ricinus in Poland and that a prevalence of spirochete-infected ticks may be high in some locations.
1996年至1998年期间，从波兰全国8个省份的25个不同地点的植被中总共采集了2285只蓖麻硬蜱（1063只若虫、637只雄蜱、585只雌蜱）。蜱虫栖息于所有25个采集地点。每个采集地点蜱虫的平均数量为91.4±13.7只。对所有2285只蜱虫进行了伯氏疏螺旋体狭义（s.l.）检测，其中来自3个省份的1333个样本通过使用多克隆抗体PAB 1B29的常规间接免疫荧光测定法（IFA）进行检测。来自5个省份的其余952个样本使用FL6和FL7引物通过聚合酶链反应（PCR）进行检测。通过这两种方法估计的蜱虫总体感染率为10.2%。与成年蜱虫相比，若虫的阳性率较低（6.2%），成年蜱虫中雌性的阳性率为14.9%，雄性为12.4%。在卡托维兹省发现感染蓖麻硬蜱的比例最高（37.5%），而在比亚韦斯托克省最低（4.1%）。在特定的采集地点，感染率在0%至37.5%之间变化。所获得的结果证实，狭义伯氏疏螺旋体在波兰蓖麻硬蜱的整个分布区域均有存在，并且在某些地点，感染螺旋体的蜱虫患病率可能很高。
