Entenmann G, Hauner H
Diabetes Research Institute, Düsseldorf, Germany.
Am J Physiol. 1996 Apr;270(4 Pt 1):C1011-6. doi: 10.1152/ajpcell.1996.270.4.C1011.
The aim of this study was to investigate the role of cell replication for the differentiation of human adipocyte precursor cells in primary culture. When cells were seeded in a medium supplemented with 10% fetal bovine serum, they started to proliferate within 48 h after exposure, as assessed by cell counting and [3H]thymidine autoradiography. When cells were inoculated in the absence of serum, a significant degree of cell proliferation was not detectable. Histochemical investigations using bromodeoxyuridine incorporation demonstrated that cells replicating their DNA did not accumulate lipid droplets. Inoculating adipocyte precursor cells under completely serum-free conditions resulted in a 30-50% higher expression of lipogenic enzymes such as glycerol-3-phosphate dehydrogenase and lipoprotein lipase than keeping cells in serum-supplemented medium for the initial 16 h. Addition of cytosine arabinoside at concentrations that effectively block mitosis did not interfere with adipocyte development. In conclusion, adipocyte precursor cells from human adipose tissue do not require cell division to enter the differentiation process in vitro. These cells may have already undergone possibly critical cell divisions in vivo and may be in a late stage of adipocyte development.
本研究的目的是调查细胞复制在原代培养的人脂肪细胞前体细胞分化过程中的作用。当细胞接种于添加10%胎牛血清的培养基中时，如通过细胞计数和[3H]胸苷放射自显影评估，它们在接种后48小时内开始增殖。当细胞在无血清条件下接种时，未检测到显著程度的细胞增殖。使用溴脱氧尿苷掺入的组织化学研究表明，复制其DNA的细胞不会积累脂滴。在完全无血清条件下接种脂肪细胞前体细胞，与在最初16小时将细胞置于补充血清的培养基中相比，导致甘油-3-磷酸脱氢酶和脂蛋白脂肪酶等生脂酶的表达高出30-50%。添加有效阻断有丝分裂浓度的阿糖胞苷并不干扰脂肪细胞的发育。总之，来自人脂肪组织的脂肪细胞前体细胞在体外进入分化过程不需要细胞分裂。这些细胞可能已经在体内经历了可能关键的细胞分裂，并且可能处于脂肪细胞发育的后期阶段。
